Atg12 (Autophagy-related Protein 12, Atg12, Apg12L, APG12-like) | US Biological A2298-75A2 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Atg12 (Autophagy-related Protein 12, Atg12, Apg12L, APG12-like)

catalog :

A2298-75A2

quantity :

100 ul

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

10E4(D88H11)

reactivity :

human

product information

Catalog Number :

A2298-75A2

Product wo Prefix :

Atg12 (Autophagy-related Protein 12, Atg12, Apg12L, APG12-like)

Host :

rabbit

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

10E4(D88H11)

Isotype :

IgG

Desc1 :

Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves an ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin-E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7,8). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:100. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Hu Mk Mo Rt

Immunogen :

Synthetic peptide corresponding to residues surrounding Ser36 of human Atg12.

Specificity :

Recognizes human endogenous levels of total free Atg 12 and Atg12 present in Atg5-Atg12 complex. Species Crossreactivity: mouse, rat, monkey.

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG Clone No: 10E4(D88H11) Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Immunogen: Synthetic peptide corresponding to residues surrounding Ser36 of human Atg12. Specificity: Recognizes human endogenous levels of total free Atg 12 and Atg12 present in Atg5-Atg12 complex. Species Crossreactivity: mouse, rat, monkey. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IP WB

Storage Temperature :

-20°C

Reference :

1. Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21, 2. Codogno, P. and Meijer, A.J. (2005) Cell Death Differ12 Suppl 2, 1509-18, 3. Levine, B. and Yuan, J. (2005) J Clin Invest 115, 2679-88, 4. Mizushima, N. et al. (1998) J Biol Chem 273, 33889-92, 5. Mizushima, N. et al. (1998) Nature 395, 395-8, 6. Suzuki, K. et al. (2001) EMBO J 20, 5971-81, 7. Tanida, I. et al. (1999) Mol Biol Cell 10, 1367-79, 8. Shintani, T. et al. (1999) EMBO J 18, 5234-41.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/37/A2298-75A2_1.jpg