antibody

a1-Antitrypsin (AAT, alpha-1-AT) (HRP) discontinued

A2298-29H

1 ml

polyclonal

domestic sheep

HRP

a1-Antitrypsin (AAT, alpha-1-AT) (HRP) discontinued

sheep

Pab

HRP

Antibodies

1 ml

IgG

Alpha-1-antitrypsin (AAT) consisting of a single polypeptide chain and has a molecular weight of 51kD. It exists in a number of genetic variants. MM variant is the most common. Alpha-1-AT is synthesized in the liver and it acts as an inhibitor of proteases such as trypsin, elastase, chymotrypsin, collagenase, leukocytic proteases, plasmin, and thrombin, which may be released during inflammatory reactions in the lung. In the absence of AAT, these enzymes are not inhibited and they may digest pulmonary parenchyma. AAT deficiency is associated with chronic obstructive lung disease (emphysema) and less frequently with hepatic cirrhosis in infants and respiratory distress of the newborn. Increase in AAT occurs as an acute phase response to tissue necrosis and inflammation. Serum level of alpha-1-AT is elevated in rheumatoid arthritis, bacterial infections, vasculitis, and carcinomatosis. In colorectal and lung carcinomas, AAT has also related to their invasive and metastatic capacity. The average level of AAT in plasma is 1.3g/L. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: 1:6400-1:12,800 Optimal working dilutions to be determined by researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu

Human alpha 1- antitrypsin, purified from plasma.

Recognizes alpha 1-antitrypsin, a 51kD glycoprotein which demonstrates serine protease inhibitor activity.

Purified by ion exchange chromatography from serum.

Supplied as a liquid in PBS, 0.05% Proclin 300, 0.002% Methylisothiazolone, 0.002% Bromonitrodioxane, 1% BSA. Labeled with horseradish peroxidase (HRP)

As reported

Product Type: Pab Isotype: IgG Host: sheep Source: human Concentration: As reported Form: Supplied as a liquid in PBS, 0.05% Proclin 300, 0.002% Methylisothiazolone, 0.002% Bromonitrodioxane, 1% BSA. Labeled with horseradish peroxidase (HRP) Purity: Purified by ion exchange chromatography from serum. Immunogen: Human alpha 1- antitrypsin, purified from plasma. Specificity: Recognizes alpha 1-antitrypsin, a 51kD glycoprotein which demonstrates serine protease inhibitor activity. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E

-20°C