beta-Amyloid | US Biological A2275-75B product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

beta-Amyloid

catalog :

A2275-75B

quantity :

100 ul

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

A2275-75B

Product wo Prefix :

beta-Amyloid

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ul

Isotype :

IgG

Desc1 :

Amyloid beta (A4) precursor protein (APP) is a 100–140kD transmembrane glycoprotein existing in several different isoforms. The amino acid sequence of APP contains the amyloid domain (A-beta), which can be released by a two-step proteolytic cleavage. The extracellular deposition and accumulation of the released A-beta fragments form the main components of amyloid plaque in Alzheimer’s disease. APP can be phosphorylated at several sites, which may affect the proteolytic processing and secretion pathway of this protein. The phosphorylation at Thr668 (at a position corresponding to the APP695 isoform) by cyclin-dependent kinase is cell cycle (G2/Mphase) dependent. The APP Thr668 phosphorylated form exists in adult rat brain and correlates with cultured neuronal differentiation. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Immunofluorescence (Paraffin): 1:1000 Immunohistochemistry (Paraffin): 1:200. Use citrate as unmasking buffer. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Immunogen :

Synthetic peptide corresponding to residues at the N-terminus of human beta-amyloid peptides.

Specificity :

Recognizes several isoforms of human beta-amyloid peptide (Abeta) such as beta-amyloid 40, beta-amyloid 42 (regardless of phosphoralytation state) and others at ~5kD.

Purity :

Purified by Protein A and peptide affinity chromatography.

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Concentration :

Not Determined

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues at the N-terminus of human beta-amyloid peptides. Specificity: Recognizes several isoforms of human beta-amyloid peptide (Abeta) such as beta-amyloid 40, beta-amyloid 42 (regardless of phosphoralytation state) and others at ~5kD. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IF IHC WB

Storage Temperature :

-20°C

Reference :

1. Selkoe, D.J. (1996) J. Biol. Chem. 271, 18295–18298. 2. Caporaso, G.L. et al. (1992) Proc. Natl. Acad. Sci. USA 89, 3055–3059. 3. Hung, A.Y. and Selkoe, D.J. (1994) EMBO J. 13, 534–542. 4. Suzuki, T. et al. (1994) EMBO J. 13, 1114–1122. 5. Ando, K. et al. (1999) J. Neurosci. 19, 4421–4427. 6. Iijima, K.I. et al. (2000) J. Neurochem. 75, 1085–1091.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/23/A2275-75B_1.jpg