antibody

Amyloid Precursor Protein (APP, Amyloid beta (A4) Precursor Protein)

A2275-69

100 ul

polyclonal

domestic rabbit

nonconjugated

Amyloid Precursor Protein (APP, Amyloid beta (A4) Precursor Protein)

rabbit

Pab

Antibodies

100 ul

IgG

Amyloid beta (A4) precursor protein (APP) is a 100-140kD transmembrane glycoprotein existing in several different isoforms (1). The amino acid sequence of APP contains the amyloid domain (A-beta), which can be released by a two-step proteolytic cleavage (1). The extracellular deposition and accumulation of the released A-beta fragments form the main components of amyloid plaque in Alzheimer’s disease (1). APP can be phosphorylated at several sites, which may affect the proteolytic processing and secretion pathway of this protein (2-5). The phosphorylation at Thr668 (at a position corresponding to the APP695 isoform) by cyclin-dependent kinase is cell cycle (G2/Mphase) dependent (4). The APP Thr668 phosphorylated form exists in adult rat brain and correlates with cultured neuronal differentiation (5,6). Applications: Suitable for use in ELISA, Western Blot and Immunofluorescence. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunofluorescence (IF-IC): 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mk Mo Rt

A4, AAA, ABETA, ABPP, AD1, Alzheimer disease amyloid protein, amyloid beta (A4) precursor protein (peptidase nexin-II, Alzheimer disease), Amyloid beta A4 protein precursor, APPI, Cerebral vascular amyloid peptide, CTFgamma, CVAP, PN2, PN-II, PreA4, Protease nexin-II

Synthetic peptide corresponding to residues surrounding Thr668 of human APP695.

Recognizes endogenous levels of several isoforms of both mature and immature human amyloid beta (A4) precursor protein, including APP695, APP770 and APP751. Mol. Wt: 100-140kD. Species Crossreactivity: monkey, mouse and rat.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined. Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues surrounding Thr668 of human APP695. Specificity: Recognizes endogenous levels of several isoforms of both mature and immature human amyloid beta (A4) precursor protein, including APP695, APP770 and APP751. Mol. Wt: 100-140kD. Species Crossreactivity: monkey, mouse and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E IF WB

-20°C

1. Selkoe, D.J. (1996) J. Biol. Chem. 271, 18295–18298. 2. Caporaso, G.L. et al. (1992) Proc. Natl. Acad. Sci. USA 89, 3055–3059. 3. Hung, A.Y. and Selkoe, D.J. (1994) EMBO J. 13, 534–542. 4. Suzuki, T. et al. (1994) EMBO J. 13, 1114–1122. 5. Ando, K. et al. (1999) J. Neurosci. 19, 4421–4427. 6. Iijima, K.I. et al. (2000) J. Neurochem. 75, 1085–1091.

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https://usbio-images.r.worldssl.net/prodimages/4/A2275-69_6.jpg

Immunohistochemical staining of paraffin-embedded Alzheimer’s brain, showing cytoplasmic localization, using A2275-69.