D-Amino Acid Oxidase (D-AAO, D-Amino acid: oxygen oxidoreductase, deaminating, DAO, OXDA) (HRP) | US Biological A1373-15 product information

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product summary

company name :

US Biological

product type :

antibody

product name :

D-Amino Acid Oxidase (D-AAO, D-Amino acid: oxygen oxidoreductase, deaminating, DAO, OXDA) (HRP)

catalog :

A1373-15

quantity :

100 ug

clonality :

polyclonal

host :

domestic sheep

conjugate :

HRP

reactivity :

pigs

product information

Catalog Number :

A1373-15

Product wo Prefix :

D-Amino Acid Oxidase (D-AAO, D-Amino acid: oxygen oxidoreductase, deaminating, DAO, OXDA) (HRP)

Host :

sheep

Product Type :

Pab

Conjugate :

HRP

Category :

Antibodies

Size1 :

100 ug

Isotype :

IgG

Desc1 :

D-Amino Acid Oxidase is a flavoprotein oxidase, flavin being the only prosthetic group. The enzyme from porcine kidney has been extensively studied. It is isolated as a stable crystalline complex with benzoate, from which the holo and apoenzyme may be prepared. The benzoate is readily exchanged for a substrate. Application: Suitable for use in Western Blot, ELISA and Immunoprecipitation. Other applications not tested. Recommended Dilution: ELISA: 1:5000-1:20,000 Western Blot: 1:500-1:5000 Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Po Sh

Immunogen :

D-Amino Acid Oxidase (Porcine Kidney).

Specificity :

Recognizes porcine D-Amino Acid Oxidase. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-sheep serum as well as purified and partially purified D-Amino Acid Oxidase (porcine kidney).

Purity :

Puriﬁed from monospeciﬁc antiserum by delipidation, fractionation and ion exchange chromatography.

Form :

Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% gentamicin sulfate. Labeled with horseradish peroxidase (HRP).

Concentration :

~1mg/ml (UV280)

Desc2 :

Product Type: Pab Isotype: IgG Host: sheep Source: porcine Concentration: ~10mg/ml (UV280) Form: Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% gentamicin sulfate. Labeled with horseradish peroxidase (HRP). Purity: Puriﬁed from monospeciﬁc antiserum by delipidation, fractionation and ion exchange chromatography. Immunogen: D-Amino Acid Oxidase (Porcine Kidney). Specificity: Recognizes porcine D-Amino Acid Oxidase. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-sheep serum as well as purified and partially purified D-Amino Acid Oxidase (porcine kidney). Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E WB

Storage Temperature :

-20°C