antibody

Akt, phosphorylated (Ser473) (Rac PKa, PKBa) (Biotin)

A1124-02P

100 ul

monoclonal

domestic rabbit

biotin

phosphorylated

10g135(D9E)

Akt, phosphorylated (Ser473) (Rac PKa, PKBa) (Biotin)

rabbit

Mab

Biotin

Phosphorylated

Antibodies

100 ul

10g135(D9E)

IgG

Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9) and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3alpha and beta (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3beta mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip (15) and p21 Waf1/CIP1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18). Inhibition of mTOR stops the protein synthesis machinery by inactivating p70 S6 kinase and activating the eukaryotic initiation factor 4E binding protein 1 (4E-BP1), an inhibitor of translation (18,19). Applications: Suitable for use in Flow Cytometry and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:400 Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Bo Hm Hu Mo Rt Ze

Synthetic phosphopeptide corresponding to residues around Ser473 of human Akt. Species sequence homology: chicken, xenopus, canine and porcine (100%)

Recognizes endogenous levels of human Akt only when phosphorylated at Ser473. Species crossreactivity: Mouse, rat, hamster, zebrafish and bovine.

Purified

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Labeled with Biotin.

Not determined

Product Type: Mab Isotype: IgG Clone No: 10g135(D9E) Host: rabbit Source: human Concentration: Not determined. Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Labeled with Biotin. Purity: Purified Immunogen: Synthetic phosphopeptide corresponding to residues around Ser473 of human Akt. Species sequence homology: chicken, xenopus, canine and porcine (100%) Specificity: Recognizes endogenous levels of human Akt only when phosphorylated at Ser473. Species crossreactivity: Mouse, rat, hamster, zebrafish and bovine. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

FC WB

-20°C

https://usbio-images.r.worldssl.net/prodimages/46/A1124-02P_1.jpg