antibody

AIF1 (Allograft Inflammatory Factor 1, AIF-1, Ionized Calcium-binding Adapter Molecule 1, Protein G1, G1, IBA1)

A1059-86G

100 ug

polyclonal

domestic goat

nonconjugated

human, mouse

AIF1 (Allograft Inflammatory Factor 1, AIF-1, Ionized Calcium-binding Adapter Molecule 1, Protein G1, G1, IBA1)

goat

Pab

Antibodies

100 ug

IgG

AIF-1 is a 17kD IFN-gamma inducible Ca2+-binding EF-hand protein, associated with chronic inflammatory processes. AIF-1 is constitutively expressed in lymphatic tissue and is up-regulated in monocytes/macrophages under various pathological conditions such as chronic rejection of allografts and in autoimmune lesions. In the brain, a subset of microglial cells constitutively express AIF-1. Increased numbers of AIF-1 immunoreactive macrophage/microglial cells were observed in focal brain infarctions, traumatic brain injury, gliomas, uveitis and in inflammatory lesions of a rat model of autoimmune disease, autoimmune encephalomyelitis and neuritis. Several Ca2+-binding peptides have been associated with monocyte activation, in particular members of the S-100 supergene-family. AIF-1 is a Ca2+-binding peptide which is not a member of the S-100 gene cluster but is located within the HLA class III genomic region. Applications: Suitable for use in Peptide ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Peptide ELISA: 1:32,000 Western Blot: 0.5-1.5ug/ml, using human brain (Frontal cortex) and human peripheral blood mononucleocyte lysates (~17kD). Immunohistochemistry (Paraffin): 2-4ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mo

Peptide corresponding to C-NKQFLDDPKYSSDED at the internal region of human AIF1 (NP_001614.3). Species Sequence Homology: canine and rat

Recognizes human AIF1. Species Crossreactivity: mouse

Purified by immunoaffinity chromatography

Supplied as a liquid in Tris saline, 0.5% BSA, pH 7.3, 0.02% sodium azide

~0.5mg/ml

Product Type: Pab Host: goat Source: human Concentration: ~0.5mg/ml Form: Supplied as a liquid in Tris saline, 0.5% BSA, pH 7.3, 0.02% sodium azide Purity: Purified by immunoaffinity chromatography. Immunogen: Peptide corresponding to C-NKQFLDDPKYSSDED at the internal region of human AIF1 (NP_001614.3). Species Sequence Homology: canine and rat Specificity: Recognizes human AIF1. Species Crossreactivity: mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E IHC WB

-20°C

Autieri MV, Agrawal N. IRT-1, a novel interferon-gamma-responsive transcript encoding a growth- suppressing basic leucine zipper protein. J Biol Chem. 1998 Jun 12;273(24):14731-7.

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