antibody

Activin Receptor Type IIA (RIIA)

A0856-05E

500 ug

monoclonal

mouse

nonconjugated

9H20

The same clone is also sold as:

• MyBioSource: MBS606695

Activin Receptor Type IIA (RIIA)

mouse

Mab

Antibodies

500 ug

9H20

IgG2a

Activin isoforms and other members of the TGF­beta superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine­threonine kinase receptor, both of which are essential for signal transduction. To date, seven type I and five type II receptors, including the two type I and the two type II activin receptors, designated ActR­I(A), ActR­IB, ActR­II(A) and ActR­IIB, have been cloned from mammals. Through alternative mRNA splicing, multiple ActR­IIB isoforms can also be generated, adding to the complexity of the activin receptor system. Different activin isoforms bind with different high­affinities to the various type II isoforms. Type I activin receptors do not bind directly to activin, but will associate with the type II receptor­activin complex and initiate signal transduction. Besides the activin isoforms, ActR­II will also bind inhibin, BMP­2 and BMP­7 with lower affinities. ActR­I can also bind and form signaling complexes with the BMP­2/7­bound BMPR­II. Activin type II receptors are highly conserved. Human, mouse and rat type II activin receptors share greater than 98% amino acid sequence homology. Recombinant soluble activin type II receptors bind activin with high affinity, and are potent activin antagonists. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml using human testis tissue. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Hu

Recombinant protein corresponding to Ala20-Pro134 of human Activin RIIA expressed in Sf21 cells.

Recognizes human Activin Receptor Type IIA (RIIA). Does not crossreact with recombinant human Activin RIA, recombinant human Activin RIB or recombinant human Activin RIIB.

Purified by Protein A affinity chromatography from hybridoma culture supernatant.

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 1ml sterile PBS.

~0.5mg/ml (after reconstitution)

Product Type: Mab Isotype: IgG2a Clone No: 9H20 Host: mouse Source: human Concentration: ~0.5mg/ml (after reconstitution) Form: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 1ml sterile PBS. Purity: Purified by Protein A affinity chromatography from hybridoma culture supernatant. Immunogen: Recombinant protein corresponding to Ala20-Pro134 of human Activin RIIA expressed in Sf21 cells. Specificity: Recognizes human Activin Receptor Type IIA (RIIA). Does not crossreact with recombinant human Activin RIA, recombinant human Activin RIB or recombinant human Activin RIIB. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E WB

-20°C

1. Attisano, L. et al. (1996) Mol. and Cell. Biol. 16:1066. 2. Woodruff, T.K. (1998) Biochem. Pharmacology, 55:953.

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