Activin Receptor Type IIA (RIIA) | US Biological A0856-05C product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Activin Receptor Type IIA (RIIA)

catalog :

A0856-05C

quantity :

100 ug

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

A0856-05C

Product wo Prefix :

Activin Receptor Type IIA (RIIA)

Host :

goat

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ug

Isotype :

IgG

Desc1 :

Members of the TGF-beta superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine-threonine kinase receptor, both of which are essential for signal transduction. Activin receptor type IIB is a type II receptor that mediates multiple signals for transforming growth factor-beta (TGF-beta) family members, including Activin, Nodal, Bmp7, Gdf1, Gdf3, Myostatin (Gdf8), and Gdf11. Through alternative mRNA splicing, multiple ActR-IIB isoforms can also be generated. Applications: Suitable for use in Neutralization, ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Neutralization: ~1-3ug/ml will block 50% of the binding of 30ng/ml recombinant human Activin A to immobilized recombinant human Act RIIA/Fc Chimera (100ul of a 0.5ng/ml solution was coated in each well) in a functional ELISA assay. Western Blot: 0.1ug/ml Immunohistochemistry: 5-15ug/ml using immersion fixed paraffin-embedded sections of human prostate cancer tissue Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Immunogen :

Recombinant protein corresponding to Ser25-Asp82 from human Activin RIIA, expressed in Sf 21 cells.

Specificity :

Recognizes human Activin Receptor Type IIA (RIIA). Shows < 2% crossreactivity with recombinant human Activin RIIB.

Purity :

Purified by immunoaffinity chromatography. Endotoxin: ≤0.1EU/ug

Form :

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.

Concentration :

~0.2mg/ml (after reconstitution)

Desc2 :

Product Type: Pab Isotype: IgG Host: goat Source: human Concentration: ~0.2mg/ml (after reconstitution) Form: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS. Purity: Purified by immunoaffinity chromatography. Endotoxin: ≤0.1EU/ug Immunogen: Recombinant protein corresponding to Ser25-Asp82 from human Activin RIIA, expressed in Sf 21 cells. Specificity: Recognizes human Activin Receptor Type IIA (RIIA). Shows < 2% crossreactivity with recombinant human Activin RIIB. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IHC N WB

Storage Temperature :

-20°C