Activation-induced Cytidine Deaminase (AICDA, AID, ARP2, CDA2, Cytidine Aminohydrolase, HIGM2, Integrated into Burkitt's Lymphoma Cell Line Ramos) | US Biological A0855-75Q product information

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product summary

company name :

US Biological

product type :

antibody

product name :

Activation-induced Cytidine Deaminase (AICDA, AID, ARP2, CDA2, Cytidine Aminohydrolase, HIGM2, Integrated into Burkitt's Lymphoma Cell Line Ramos)

catalog :

A0855-75Q

quantity :

100 ul

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

10g132(30F12)

reactivity :

human

product information

Catalog Number :

A0855-75Q

Product wo Prefix :

Activation-induced Cytidine Deaminase (AICDA, AID, ARP2, CDA2, Cytidine Aminohydrolase, HIGM2, Integrated into Burkitt's Lymphoma Cell Line Ramos)

Host :

rabbit

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

10g132(30F12)

Isotype :

IgG

Desc1 :

Activation-induced cytidine deaminase (AID) is thought to modify RNA due to its high homology to the RNA editing enzyme APOBEC-1. This function, however, has not been confirmed in in vitro studies, which show that AID has significant cytidine deaminase activity, and that this activity is blocked by zinc chelation.The B cell immune system must specifically recognize several infectious agents, which vastly outnumber immunoglobulin gene segments present in a given organism. Mechanisms such as somatic hypermutation, isotype switch recombination and gene conversion introduce diversity and specificity to the immune system. Analysis of mouse models and patients with AID deficiency has established a link between all three of these mechanisms and AID function. AID protein is detected in germinal center centroblast and germinal center derived lymphomas (Burkitt lymphoma), but not in pre-germinal center B cells or post-germinal center neoplasms (B cell chronic lymphocytic leukemia and multiple myeloma). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000. Incubate membrane with diluted antibody in 1X TBS, 5% BSA, 0.1% Tween-20 at 4ºC with gentle shaking, overnight. Immunoprecipitation: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Alternate Name :

EC=3.5.4.5

Immunogen :

Synthetic peptide corresponding to residues surrounding Leu165 of human AID.

Specificity :

Recognizes endogenous levels of total human AID protein.

Purity :

Purified

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Concentration :

Not Determined

Desc2 :

Product Type: Mab Isotype: IgG Clone No: 10g132(30F12) Host: rabbit Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to residues surrounding Leu165 of human AID. Specificity: Recognizes endogenous levels of total human AID protein. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

IP WB

Storage Temperature :

-20°C

Reference :

1. Muramatsu, M. et al. (1999) J. Biol. Chem. 274, 18470-18476. 2. Reynaud, C.A. et al. (2003) Nat. Immunol. 7, 631-638. 3. Pasqualucci, L. et al. (2004) Blood 104, 3318-3325.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/14/A0855-75Q_1.jpg