Activation-induced Cytidine Deaminase (AICDA, AID, ARP2, CDA2, HIGM2) | US Biological A0855-75A product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Activation-induced Cytidine Deaminase (AICDA, AID, ARP2, CDA2, HIGM2)

catalog :

A0855-75A

quantity :

100 ul

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

7H3(EK2 5G9)

reactivity :

human

product information

Catalog Number :

A0855-75A

Product wo Prefix :

Activation-induced Cytidine Deaminase (AICDA, AID, ARP2, CDA2, HIGM2)

Host :

rat

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

7H3(EK2 5G9)

Isotype :

IgG2b,k

Desc1 :

Activation-induced cytidine deaminase (AID) is thought to modify RNA due to its high homology to the RNA editing enzyme APOBEC-1. This function, however, has not been confirmed in in vitro studies, which show that AID has significant cytidine deaminase activity, and that this activity is blocked by zinc chelation. The B cell immune system must specifically recognize several infectious agents, which vastly outnumber immunoglobulin gene segments present in a given organism. Mechanisms such as somatic hypermutation, isotype switch recombination and gene conversion introduce diversity and specificity to the immune system. Analysis of mouse models and patients with AID deficiency has established a link between all three of these mechanisms and AID function. AID protein is detected in germinal center centroblast and germinal center derived lymphomas (Burkitt lymphoma), but not in pre-germinal center B cells or post-germinal center neoplasms (B cell chronic lymphocytic leukemia and multiple myeloma). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-49U: IgG, F(ab’)2, H&L X-Adsorbed (FITC) RbxRt Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Immunogen :

Synthetic peptide corresponding to a region surrounding Asp191 of human AID.

Specificity :

Recognizes endogenous levels of total human AID protein at ~24kD.

Purity :

Ascites

Form :

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Concentration :

Not Determined

Desc2 :

Product Type: Mab Isotype: IgG2b,k Clone No: 7H3(EK2 5G9) Host: rat Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Ascites Immunogen: Synthetic peptide corresponding to a region surrounding Asp191 of human AID. Specificity: Recognizes endogenous levels of total human AID protein at ~24kD. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

Storage Temperature :

-20°C

Reference :

1.Muramatsu, M. et al. (1999) J. Biol. Chem. 274: 18470–18476. 2. Reynaud, C.A. et al. (2003) Nat. Immunol. 7: 631–638. 3. Pasqualucci, L. et al. (2004) Blood 104: 3318–3325.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/20/A0855-75A_1.jpg

Picture 6 File Name :

https://usbio-images.r.worldssl.net/prodimages/20/A0855-75A_6.jpg

Picture6 Text :

Flow Cytometry analysis of Raji cells using using A0855-75A (blue) compared to a nonspecific negative control antibody (red).