antibody

Activation Transcription Factor 2, phosphorylated (Thr71) (ATF2, CREB P1) discontinued

A0855-42B

100 ul

polyclonal

domestic rabbit

nonconjugated

phosphorylated

Activation Transcription Factor 2, phosphorylated (Thr71) (ATF2, CREB P1) discontinued

rabbit

Pab

Phosphorylated

Antibodies

100 ul

IgG

The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins. ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways. Various forms of cellular stress, including genotoxic agents, inflammatory cytokines and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71. Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress-induced transcription by ATF-2. In addition, mutations at these sites also reduce the ability of E1A and Rb to stimulate gene expression via ATF-2. Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunoprecipitation: 1:250 Immunohistochemistry (paraffin/frozen): 1:50 Immunofluorescence (IF/IC): 1:50 Flow Cytometry: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Mk Mo Rt

Synthetic phosphopeptide corresponding to residues surrounding Thr71 of human ATF2.

Recognizes endogenous levels of human ATF-2 only when phosphorylated at threonine 71. Does not cross-react with phosphorylated c-Jun, CREB or other transcription factors. Recognizes both Thr69/Thr71 dually phosphorylated ATF-2 and Thr71 singly phosphorylated ATF-2 equally well. Species Crossreactivity: mouse, rat and monkey

Purified by Protein A and peptide affinity chromatography.

Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Thr71 of human ATF2. Specificity: Recognizes endogenous levels of human ATF-2 only when phosphorylated at threonine 71. Does not cross-react with phosphorylated c-Jun, CREB or other transcription factors. Recognizes both Thr69/Thr71 dually phosphorylated ATF-2 and Thr71 singly phosphorylated ATF-2 equally well. Species Crossreactivity: mouse, rat and monkey Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

FC IC IF IHC IP WB

-20°C

1. Abdel-Hafiz, H. et al. (1992) Mol. Endocrinol. 6, 2079–2089. 2. Gupta, S. et al. (1995) Science 267, 389–393. 3. van Dam, H. et al. (1995) EMBO J. 14(8), 1798–1810. 4. Livingstone, C. et al. (1995) EMBO J. 14, 1785–1797. 5. deRuiter, N.D. et al. (2000) Mol. Cell. Biol. 20, 8480–8488. 6. Hocevar, B.A. et al. (1999) EMBO J. 18, 1345–1356. 7. Marinissen, M.J. et al. (2001) Genes Dev. 15, 535–553. 8. Ouwens, D.M. et al. (2002) EMBO J. 21, 3782–3793. 9. Suh, Y. et al. (2000) Cancer Res. 60, 5067–5073. 10. Zhang, S. and Kaplan, M.H. (2000) J. Immunol. 165, 1374–1380.

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