antibody

Actin, beta (ACTB, Actin Cytoplasmic 1, Beta Cytoskeletal Actin) (HRP)

A0760-41L

100 ul

monoclonal

domestic rabbit

HRP

10g129(13E5)

Actin, beta (ACTB, Actin Cytoplasmic 1, Beta Cytoskeletal Actin) (HRP)

rabbit

Mab

HRP

Antibodies

100 ul

10g129(13E5)

IgG

Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle b- and g-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility. a-cardiac anda-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, a- and g-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells. Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin. The Arp2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments. It has been reported that actin is hyperphosphorylated in primary breast tumors. Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle. Actin cleavage by caspase-3 may accelerate ubiquitin/proteosome dependent muscle proteolysis. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.

Bo Hu Mk Mo Po Rt

Synthetic peptide corresponding residues near the N-terminal of human b-actin (P60709). Species Sequence Homology: chicken and equine

Recognizes endogenous levels of total human beta-actin protein. May crossreact with gamma-actin (cytoplasmic isoform). Does not crossreact with alpha-skeletal, alpha-cardiac, alpha-vascular smooth muscle, or gamma-enteric smooth muscle isoforms. Species Crossreactivity: mouse, rat, monkey, bovine, and porcine

Purified

Supplied as a liquid in 136mM sodium chloride 2.6mM potassium chloride 12mM sodium phosphate dibasic, pH 7.4, 2mg/ml BSA, 50% glycerol. Labeled with horseradish peroxidase (HRP).

Not Determined

Product Type: Mab Isotype: IgG Clone No: 10g129(13E5) Host: rabbit Source: human Concentration: Not Determined Form: Supplied as a liquid in 136mM sodium chloride 2.6mM potassium chloride 12mM sodium phosphate dibasic, pH 7.4, 2mg/ml BSA, 50% glycerol. Labeled with horseradish peroxidase (HRP). Purity: Purified Immunogen: Synthetic peptide corresponding residues near the N-terminal of human b-actin (P60709). Species Sequence Homology: chicken and equine Specificity: Recognizes endogenous levels of total human beta-actin protein. May crossreact with gamma-actin (cytoplasmic isoform). Does not crossreact with alpha-skeletal, alpha-cardiac, alpha-vascular smooth muscle, or gamma-enteric smooth muscle isoforms. Species Crossreactivity: mouse, rat, monkey, bovine, and porcine Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

WB

-20°C

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