antibody

Actin, beta (ACTB, Actin Cytoplasmic 1, Beta Cytoskeletal Actin) (Biotin)

A0760-41K

100 ul

monoclonal

domestic rabbit

biotin

10g129(13E5)

Actin, beta (ACTB, Actin Cytoplasmic 1, Beta Cytoskeletal Actin) (Biotin)

rabbit

Mab

Biotin

Antibodies

100 ul

10g129(13E5)

IgG

Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle b- and g-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). a-cardiac anda-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, a- and g-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells (1). Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin (2). The Arp2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments (2). It has been reported that actin is hyperphosphorylated in primary breast tumors (3). Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle (4-6). Actin cleavage by caspase-3 may accelerate ubiquitin/proteosome dependent muscle proteolysis (6). Applications: Suitable for use in Flow Cytometry and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:200 Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Bo Hu Mk Mo Po Rt

Synthetic peptide corresponding to residues near the N-terminus of human b-actin. Species Sequence Homology: hamster, chicken, canine and equine (100%)

Recognizes endogenous levels of total beta-actin protein. This antibody may cross-react with the gamma-actin (cytoplasmic isoform). It does not cross-react with alpha-skeletal, alpha-cardiac, alpha-vascular smooth, or gamma-enteric smooth muscle isoforms. Species crossreactivity: Mouse, rat, monkey, bovine and porcine.

Purified

Supplied as a liquid in 136mM sodium chloride, 2.6mM potassium chloride, 12mM sodium phosphate pH 7.4 dibasic, 2mg/ml BSA, 50% glycerol. Labeled with Biotin.

Not determined

Product Type: Mab Isotype: IgG Clone No: 10g129(13E5) Host: rabbit Source: human Concentration: Not determined. Form: Supplied as a liquid in 136mM sodium chloride, 2.6mM potassium chloride, 12mM sodium phosphate pH 7.4 dibasic, 2mg/ml BSA, 50% glycerol. Labeled with Biotin. Purity: Purified Immunogen: Synthetic peptide corresponding to residues near the N-terminus of human b-actin. Species Sequence Homology: hamster, chicken, canine and equine (100%) Specificity: Recognizes endogenous levels of total beta-actin protein. This antibody may cross-react with the gamma-actin (cytoplasmic isoform). It does not cross-react with alpha-skeletal, alpha-cardiac, alpha-vascular smooth, or gamma-enteric smooth muscle isoforms. Species crossreactivity: Mouse, rat, monkey, bovine and porcine. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

FC WB

-20°C

https://usbio-images.r.worldssl.net/prodimages/33/A0760-41K_1.jpg