Actin, beta (ACTB, Actin Cytoplasmic 1, Beta Cytoskeletal Actin) | US Biological A0760-41J product information

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product summary

company name :

US Biological

product type :

antibody

product name :

Actin, beta (ACTB, Actin Cytoplasmic 1, Beta Cytoskeletal Actin)

catalog :

A0760-41J

quantity :

100 ul

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

10g129(13E5)

reactivity :

human

product information

Catalog Number :

A0760-41J

Product wo Prefix :

Actin, beta (ACTB, Actin Cytoplasmic 1, Beta Cytoskeletal Actin)

Host :

rabbit

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

10g129(13E5)

Isotype :

IgG

Desc1 :

Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle b- and g-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). a-cardiac anda-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, a- and g-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells (1). Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin (2). The Arp2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments (2). It has been reported that actin is hyperphosphorylated in primary breast tumors (3). Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle (4-6). Actin cleavage by caspase-3 may accelerate ubiquitin/proteosome dependent muscle proteolysis (6). Applications: Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence (IF-IC): 1:100. Methanol permeabilization is required. Flow Cytometry: 1:100 Western Blot: 1:1000 Immunohistochemistry (Paraffin): 1:200. Antigen retrieval with citrate buffer is recommended. Immunohistochemistry (Frozen): 1:200. Fixation in 10% Neutral buffered formalin is recommended. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Bo Hu Mk Mo Po Rt

Immunogen :

Synthetic peptide corresponding to residues near the N-terminal residues of human beta-actin. Species Sequence Homology: chicken, canine, equine.

Specificity :

Recognizes endogenous levels of total human beta-actin protein at ~45kD. May crossreact with the gamma-actin (cytoplasmic isoform). Does not crossreact with alpha-skeletal, alpha-cardiac, alpha-vascular smooth or gamma-enteric smooth muscle isoforms. Species Crossreactivity: mouse, rat, monkey, bovine, porcine.

Purity :

Purified

Form :

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG Clone No: 10g129(13E5) Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to residues near the N-terminal residues of human beta-actin. Species Sequence Homology: chicken, canine, equine. Specificity: Recognizes endogenous levels of total human beta-actin protein at ~45kD. May crossreact with the gamma-actin (cytoplasmic isoform). Does not crossreact with alpha-skeletal, alpha-cardiac, alpha-vascular smooth or gamma-enteric smooth muscle isoforms. Species Crossreactivity: mouse, rat, monkey, bovine, porcine. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

FC IC IF IHC WB

Storage Temperature :

-20°C

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/24/A0760-41J_1.jpg

Picture4 Text :

Flow cytometric analysis of NIH/3T3 cells using A0760-41J (blue) compared to a nonspecific negative control antibody (red).

Picture 5 File Name :

https://usbio-images.r.worldssl.net/prodimages/24/A0760-41J_5.jpg

Picture5 Text :

Confocal immunofluorescent analysis of COS-7 cells using A0760-41J (green). Blue pseudocolor= DRAQ5 fluorescent DNA dye

Picture 6 File Name :

https://usbio-images.r.worldssl.net/prodimages/24/A0760-41J_6.jpg

Picture6 Text :

Immunohistochemical analysis of paraffin-embedded 4T1 syngeneic mouse tumor using A0760-41J.

Picture 7 File Name :

https://usbio-images.r.worldssl.net/prodimages/24/A0760-41J_7.pct

Picture7 Text :

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using A0760-41J(left) or beta-Actin Blocking Peptide (right).