antibody

Actin, beta (ACTB, Actin Cytoplasmic 1, Beta Cytoskeletal Actin)

A0760-41H

20 ul

monoclonal

mouse

nonconjugated

10g128(8H10D10)

Actin, beta (ACTB, Actin Cytoplasmic 1, Beta Cytoskeletal Actin)

mouse

Mab

Antibodies

20 ul

10g128(8H10D10)

IgG2b

Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle b- and gamma-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). a-cardiac and a-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, a- and b-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells (1). Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin (2). The Arp2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments (2). It has been reported that actin is hyperphosphorylated in primary breast tumors (3). Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle (4-6). Actin cleavage by caspase-3 may accelerate ubiquitin/proteosome dependent muscle proteolysis (6). Applications: Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence (IF-IC): 1:2400 Flow Cytometry: 1:400 Western Blot: 1:1000, incubate membrane with diluted primary antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween 20 at 4°C with gentle shaking, overnight. Immunohistochemistry (paraffin): 1:4000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Ca Hm Hu Mk Mo Rt

Synthetic peptide corresponding to N-terminal residues of human beta-actin.

Recognizes endogenous levels of human total beta-Actin. Species Crossreactivity: hamster, mouse, rat, monkey and canine

Ascites

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.

Not determined

Product Type: Mab Isotype: IgG2b Clone No: 10g128(8H10D10) Host: mouse Source: human Concentration: Not determined. Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol. Purity: Ascites Immunogen: Synthetic peptide corresponding to N-terminal residues of human beta-actin. Specificity: Recognizes endogenous levels of human total beta-Actin. Species Crossreactivity: hamster, mouse, rat, monkey and canine Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

FC IF IHC WB

-20°C

https://usbio-images.r.worldssl.net/prodimages/6/A0760-41H_1.jpg

Flow cytometric analysis of Hela cells using A0760-41H.(blue) compared to a nonspecific negative control antibody (red)

https://usbio-images.r.worldssl.net/prodimages/6/A0760-41H_5.jpg

Confocal immunofluorescent analysis of A-172 cells using A0760-41H. (green) showing colocalization with actin filaments that have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye)