Actin, beta (ACTB, Actin Cytoplasmic 1, Beta Cytoskeletal Actin) discontinued | US Biological A0760-40T product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Actin, beta (ACTB, Actin Cytoplasmic 1, Beta Cytoskeletal Actin) discontinued

catalog :

A0760-40T

quantity :

50 ug

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

A0760-40T

Product wo Prefix :

Actin, beta (ACTB, Actin Cytoplasmic 1, Beta Cytoskeletal Actin) discontinued

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

50 ug

Isotype :

IgG

Desc1 :

Actins are highly conserved proteins that are involved in cell motility, structure and integrity and are ubiquitously expressed in all eukaryotic cells. Higher vertebrates express six actin isoforms encoded for by distinct genes. Actin isoforms are highly evolutionarily conserved. Two striated muscle isoforms, alpha skeletal and alpha cardiac, two smooth muscle isoforms, alpha smooth and gamma smooth together with two cytoplasmic isoforms, beta cytoplasmic and gamma cytoplasmic are encoded by genes on chromosomes 1, 15, 10, 2, 7 and 17 respectively. All actin isoforms have highly homologous nucleotide sequences with the heterogeneity most notably in the N terminal region. However, small differences in primary structure here and elsewhere affect the conformational state of the various isoforms and their affinity for a range of actin binding proteins (ABPs). The range of affinities for ABPs suggests that where multiple globular monomeric (G-actin) forms exist at the same site, polymerization may result in the formation of filamentous (F-actin) enriched for one particular isoform. Defects in actin beta are a cause of juvenile dystonia, a condition that results in movement disorders due to disordered muscle tonicity, abnormal posture, development malformations and sensory hearing loss. Applications: Suitable for use in ELISA, Immunofluorescence, Immunohistochemistry (paraffin), and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:10,000-1:40,000 Immunohistochemistry (paraffin): 5ug/ml Western Blot: 1:500-1:2000, detects a band of ~42kD in Human embryonic Kidney 293 cell lysates. Immunofluorescence: 1:500-1:4000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Immunogen :

Synthetic Peptide corresponding to aa359-368 of human beta actin. Species sequence homology: broad.

Specificity :

Recognizes human ACTIN BETA.

Purity :

Purified by immunoaffinity chromatography

Form :

Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.

Concentration :

~1mg/ml

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: ~1mg/ml Form: Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. Purity: Purified by immunoaffinity chromatography. Immunogen: Synthetic Peptide corresponding to aa359-368 of human beta actin. Uniprot/Accession Number: P60709. Species sequence homology: broad. Specificity: Recognizes human ACTIN BETA. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IF IHC WB

Storage Temperature :

-20°C

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/6/A0760-40T_1.jpg