antibody

Actin, beta

A0760-40Q

100 ul

polyclonal

domestic rabbit

nonconjugated

Actin, beta

rabbit

Pab

Antibodies

100 ul

IgG

Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle beta- and gamma-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). alpha-cardiac and alpha-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, alpha- and gamma-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate contractile potentials for muscle cells (1). Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin (2). The Arp2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments (2). It has been reported that actin is hyperphosphorylated in primary breast tumors (3). Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle (4-6). Actin cleavage by caspase-3 may accelerate ubiquitin/proteosome dependent muscle proteolysis (6). Applications: Suitable for use in Western Blot. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:1000, incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Bo Dr Hm Hu Mk Mo Rt

Synthetic peptide corresponding to N-terminal residues of human beta-actin. Species Sequence Homology: porcine, chicken, xenopus, canine.

Recognizes endogenous levels of human beta-actin at ~45kD. May crossreact with the gamma-actin (cytoplasmic) isoform. Does not crossreact with alpha-skeletal, alpha-cardiac, alpha-vascular smooth or gamma-enteric smooth muscle isoforms. Species crossreactivity: mouse, rat, monkey, mink, hamster, bovine, Drosophila, zebrafish.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to N-terminal residues of human beta-actin. Species Sequence Homology: porcine, chicken, xenopus, canine. Specificity: Recognizes endogenous levels of human beta-actin at ~45kD. May crossreact with the gamma-actin (cytoplasmic) isoform. Does not crossreact with alpha-skeletal, alpha-cardiac, alpha-vascular smooth or gamma-enteric smooth muscle isoforms. Species crossreactivity: mouse, rat, monkey, mink, hamster, bovine, Drosophila, zebrafish. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

WB

-20°C

Herman, I.M. (1993) Curr. Opin. Cell Biol. 5, 48-55.Condeelis, J. (2001) Trends Cell Biol. 11, 288-293.Lim, Y.P. et al. (2004) Clin. Cancer Res. 10, 3980-3987.Kayalar, C. et al. (1996) Proc. Natl. Acad. Sci. USA. 93, 2234-2238.Communal, C. et al. (2002) Proc. Natl. Acad. Sci. USA. 99, 6252-6256.Du, J. et al. (2004) J. Clin. Invest. 113, 115-123.

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