Acetylcholinesterase (ACHE, AChE) | US Biological A0526-20C product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Acetylcholinesterase (ACHE, AChE)

catalog :

A0526-20C

quantity :

100 ug

clonality :

polyclonal

host :

domestic goat

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

A0526-20C

Product wo Prefix :

Acetylcholinesterase (ACHE, AChE)

Host :

goat

Product Type :

Pab

Category :

Antibodies

Size1 :

100 ug

Isotype :

IgG

Desc1 :

Acetylcholinesterase (AChE, EC.3.1.1.7.) is an enzyme located in the postsynaptic membrane and in the muscle endplates, where it hydrolyses the neurotransmitter acetylcholin. AChE from brain is a tetramer (G4-AChE) with a molecular mass of 320kD, AChE from erythrocytes is a dimer (G2-AChE) with a molecular mass of 170kD. Detection of higher levels of AChE in amniotic fluid can indicate fetal malformations such as neural tube defects. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:2000 Western Blot: 1-3ug/ml, observed in human brain (cerebellum) lysates on ~70kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Bo Hu Mo Rt

Immunogen :

Synthetic peptide corresponding to QFDHYSKQDRCSDL, from human ACHE, at C-terminal (NP_000656.1).

Specificity :

Recognizes human ACHE. Species sequence homology: bovine, mouse and rat.

Purity :

Purified by immunoaffinity chromatography

Form :

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

Concentration :

~0.5mg/ml

Desc2 :

Product Type: Pab Host: goat Source: human Concentration: ~0.5mg/ml Form: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA. Purity: Purified by immunoaffinity chromatography. Immunogen: Synthetic peptide corresponding to QFDHYSKQDRCSDL, from human ACHE, at C-terminal (NP_000656.1). Specificity: Recognizes human ACHE. Species sequence homology: bovine, mouse and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E WB

Storage Temperature :

-20°C

Reference :

1. Cottingham MG, Voskuil JL, Vaux DJ. The intact human acetylcholinesterase C-terminal oligomerization domain is alpha-helical in situ and in isolation, but a shorter fragment forms beta-sheet-rich amyloid fibrils and protofibrillar oligomers. Biochemistry. 2003 Sep 16;42(36):10863-73

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/47/A0526-20C_1.jpg