antibody

Acetyl CoA Carboxylase (ACC1)

A0224

100 ul

polyclonal

domestic rabbit

nonconjugated

Acetyl CoA Carboxylase (ACC1)

rabbit

Pab

Antibodies

100 ul

IgG

Acetyl-CoA carboxylase (ACC) catalyzes the pivotal step of the fatty acid synthesis pathway. The 265kD ACCa is the predominant isoform in liver, adipocytes and mammary gland, while the 280kD ACCb is the major isoform in skeletal muscle and heart. Phosphorylation by AMPK at Ser79, or by PKA at Ser1200, inhibits the enzymatic activity of ACC. ACC is a potential target of anti-obesity drugs. Applications: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunofluorescence/Immunocytochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:50 Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Immunohistochemistry (Paraffin): 1:50. Antigen retrieval with citrate buffer is recommended. Immunoprecipitation: 1:50 Immunofluorescence (IC): 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Bo Hu Mk Mo Rt

Synthetic peptide corresponding to residues surrounding Ser523 of human acetyl CoA carboxylase a1. MW of Antigen: 280kD. Species Sequence Homology: chicken and Drosophila 100%.

Recognizes endogenous levels of all isoforms of human acetyl CoA carboxylase protein at ~280kD. Species Crossreactivity: bovine, mouse, rat and monkey.

Purified by Protein A and peptide affinity chromatography.

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Not Determined

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: Not Determined Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. Purity: Purified by Protein A and peptide affinity chromatography. Immunogen: Synthetic peptide corresponding to residues surrounding Ser523 of human acetyl CoA carboxylase a1. MW of Antigen: 280kD. Species Sequence Homology: chicken and Drosophila 100%. Specificity: Recognizes endogenous levels of all isoforms of human acetyl CoA carboxylase protein at ~280kD. Species Crossreactivity: bovine, mouse, rat and monkey. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

FC IF IHC IP WB

-20°C

1. Jianqiang M, et al. PNAS 100:7515-7520, 2003 2. Girad J, et al. The FASEB Journal 8:36-42, 1994 3. Wakil SJ, et al. Annu Rev Biochem 52:537–579, 1983 4. Abu-Elheiga L, et al. Science 291: 2613-2616. 200 5. Ruderman, N.B. et al., Am. J. Physiol. 276, E1–E18, 1999 6. Ha, J. et al., J. Biol. Chem. 269, 22162–22168, 1994 7. Abu-Elheiga, L. et al., Science 291, 2613–2616, 2001 8. Levert, K.L. et al,. J. Biol. Chem. 277, 16347–16350, 2002

https://usbio-images.r.worldssl.net/prodimages/21/A0224_1.jpg

Confocal microscopic images of A431 cells showing cytoplasmic stain with A0224 (A) compared to an isotype control (B).