Acetyl CoA Carboxylase (ACC1) | US Biological A0224-01A product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Acetyl CoA Carboxylase (ACC1)

catalog :

A0224-01A

quantity :

100 ul

clonality :

monoclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

7H2(C83B10)

reactivity :

human

product information

Catalog Number :

A0224-01A

Product wo Prefix :

Acetyl CoA Carboxylase (ACC1)

Host :

rabbit

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

7H2(C83B10)

Isotype :

IgG

Desc1 :

Acetyl-CoA carboxylase (ACC) catalyzes the pivotal step of the fatty acid synthesis pathway. The 265kD ACC alpha is the predominant isoform in liver, adipocytes and mammary gland, while the 280kD ACC beta is the major isoform in skeletal muscle and heart (1). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (2). ACC is a potential target of anti-obesity drugs (3,4). Applications: Suitable for use in ELISA, Immunofluorescence, Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Immunohistochemistry (Paraffin): 1:100 Citrate/TBST Immunofluorescence (IF-IC): 1:200 Flow Cytometry: 1:400 Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb I1904-46Q: IgG, H&L (HRP) Pab Gt xRb Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Calc Crossreactivity :

Hm Hu Mo Rt

Immunogen :

Synthetic peptide corresponding to residues surrounding Ser523 of human acetyl-CoA carboxylase alpha1.

Specificity :

Detects endogenous levels of all isoforms of acetyl-CoA carboxylase protein. Species Crossreactivity: Human, hamster, mouse and rat.

Purity :

Purified

Form :

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 0.02% sodium azide, 50% glycerol.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG Clone No: 7H2(C83B10) Host: rabbit Source: human Concentration: Not determined Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 0.02% sodium azide, 50% glycerol. Purity: Purified Immunogen: Synthetic peptide corresponding to residues surrounding Ser523 of human acetyl-CoA carboxylase alpha1. Specificity: Detects endogenous levels of all isoforms of acetyl-CoA carboxylase protein. Species Crossreactivity: Human, hamster, mouse and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E FC IF IHC WB

Storage Temperature :

-20°C

Reference :

1. Ruderman, N.B. et al. (1999) Am. J. Physiol. 276: E1–E18. 2. Ha, J. et al. (1994) J. Biol. Chem. 269: 22162–22168. 3. Abu-Elheiga, L. et al. (2001) Science 291: 2613–2616. 4. Levert, K.L. et al. (2002) J. Biol. Chem. 277: 16347-16350

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/4/A0224-01A_1.jpg

Picture4 Text :

Confocal immunofluorescent analysis of NIH/3T3 cells using A0224-01A (red). Blue pseudocolor=Draq5™ (fluorescent DNA dye).