This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
US Biological
product type :
antibody
product name :
Tyrosinase (TYR)
catalog :
138600
quantity :
20 ug
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
T311

The same clone is also sold as:
reactivity :
human
product information
Catalog Number :
138600
Product wo Prefix :
Tyrosinase (TYR)
Host :
mouse
Product Type :
Mab
Category :
Antibodies
Size1 :
20 ug
Clone # USB :
T311
Isotype :
IgG2a,k
Desc1 :
Recognizes a cluster of proteins between 70-80kD, identified as tyrosinase. Occasionally a minor band at 55kD is also detected. This monoclonal antibody shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway; the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this MAb shows tyrosinase in melanotic as well as amelanotic variants. This monoclonal antibody is a useful marker for melanocytes and melanomas. Applications: Suitable for use in ELISA, Flow Cytometry, Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug/10e6 cells Immunofluorescence: 1-2ug/ml Western Blot: 0.5-1ug/ml Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT Immunoprecipitation: 1-2ug/500ug protein lysate Optimal dilutions to be determined by the researcher. Positive Control: SK-MEL-13, SK-MEL-19, SK-MEL-30, SK-MEL-37 cells or Melanoma. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Calc Crossreactivity :
Hu
Immunogen :
Recombinant protein corresponding to tyrosinase.
Specificity :
Recognizes human tyrosinase, 70-80kD. Does not cross-react with MAGE-1 and tyrosinase-related protein-1, TRP-1/gp75. Staining of melanomas showed tyrosinase in melanotic as well as amelanotic variants.
Purity :
Purified by Protein G affinity chromatography.
Form :
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138600A.
Concentration :
~0.2mg/ml
Desc2 :
Product Type: Mab Isotype: IgG2a,k Clone No: T311 Host: mouse Source: human Concentration: ~0.2mg/ml Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138600A. Purity: Purified by Protein G affinity chromatography. Immunogen: Recombinant protein corresponding to tyrosinase. Specificity: Recognizes human tyrosinase, 70-80kD. Does not cross-react with MAGE-1 and tyrosinase-related protein-1, TRP-1/gp75. Staining of melanomas showed tyrosinase in melanotic as well as amelanotic variants. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
E FC IF IHC IP WB
Storage Temperature :
-20°C
Reference :
1. Chen Y-T, et. al. Proc. Natl. Acad. Sci. USA, 1995, 92:8125-8129.
Picture 1 File Name :
https://usbio-images.r.worldssl.net/prodimages/13/138600_1.jpg
company information
US Biological
4 Technology Way
Salem, MA01970
service@usbio.net
https://www.usbio.net
800-520-3011
headquarters: USA
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