TAG-72 (Tumor-associated Glycoprotein 72, TAG 72) | US Biological 138595 product information

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product summary

company name :

US Biological

product type :

antibody

product name :

TAG-72 (Tumor-associated Glycoprotein 72, TAG 72)

catalog :

138595

quantity :

20 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

B72.3

reactivity :

roundworm

product information

Catalog Number :

138595

Product wo Prefix :

TAG-72 (Tumor-associated Glycoprotein 72, TAG 72)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

20 ug

Clone # USB :

B72.3

Isotype :

IgG1,k

Desc1 :

Recognizes an oncofetal antigen of 220kD, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This monoclonal antibody defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80% sensitivity and 93% specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of anti-TAG-72 and anti-GCDFP-15 is valuable in the diagnosis of apocrine carcinoma. Applications: Suitable for use in ELISA, Western Blot, Flow Cytometry, Immunoprecipitation, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug/10e6 cells Western Blot: 0.5-1ug/ml Immunofluorescence: 1-2ug/ml Immunoprecipitation: 1-2ug/500ug protein lysate Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT Optimal dilutions to be determined by the researcher. Positive Control: Jurkat cells, Breast or lung carcinoma. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Bo Ca Hm Hu Rt

Immunogen :

Membrane-enriched fraction of a human breast carcinoma liver metastasis

Specificity :

Recognizes human TAG-72. Species Crossreactivity: bovine, canine, hamster and rat. Stains the majority of human adenocarcinomas including colorectal, pancreatic, gastric, ovarian, endometrial, mammary and non-small cell lung cancer.

Purity :

Purified by Protein G affinity chromatography.

Form :

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138595-AF.

Concentration :

~0.2mg/ml

Desc2 :

Product Type: Mab Isotype: IgG1,k Clone No: B72.3 Host: mouse Source: human Concentration: ~0.2mg/ml Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138595AF. Purity: Purified by Protein G affinity chromatography. Immunogen: Membrane-enriched fraction of a human breast carcinoma liver metastasis Specificity: Recognizes human TAG-72. Species Crossreactivity: bovine, canine, hamster and rat. Stains the majority of human adenocarcinomas including colorectal, pancreatic, gastric, ovarian, endometrial, mammary and non-small cell lung cancer. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E FC IF IHC IP WB

Storage Temperature :

-20°C

Reference :

1. Lottich SC et. al. Breast Cancer Research and Treatment, 1985, 6(1):49-56. 2. Thor A et. al. Cancer Research, 1986, 46(6):3118-24.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/34/138595_1.jpg