This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
US Biological
product type :
antibody
product name :
Renal Cell Carcinoma (RCC)
catalog :
138592
quantity :
20 ug
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
66.4.C2, PN-15

The same clone is also sold as:
reactivity :
human
product information
Catalog Number :
138592
Product wo Prefix :
Renal Cell Carcinoma (RCC)
Host :
mouse
Product Type :
Mab
Category :
Antibodies
Size1 :
20 ug
Clone # USB :
66.4.C2, PN-15
Isotype :
IgG2b,k
Desc1 :
Recognizes a glycoprotein of ~200kD, identified as carbonic anhydrase IX (CAIX/gp200). Its epitope resides in the carbohydrate domain of gp200. It shows no significant cross-reactivity with other carbohydrate determinants, such as the Lewis blood group antigens, epithelial membrane antigen, HMFG, and AB blood group antigens. In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, gp200 is expressed by 93% of primary and 84% of metastatic renal cell carcinomas. This monoclonal antibody may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation. Applications: Suitable for use in ELISA, Western Blot, Flow Cytometry, Immunoprecipitation, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug/10e6 cells Western Blot: 0.5-1ug/ml Immunofluorescence: 1-2ug/ml Immunoprecipitation: 1-2ug/500ug protein lysate Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT Optimal dilutions to be determined by the researcher. Positive Control: Normal kidney or renal cell carcinoma Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Calc Crossreactivity :
Eq Hu
Immunogen :
Microsomal fraction of human renal cortical tissue homogenate
Specificity :
Recognizes human Renal Cell Carcinoma. Species Crossreactivity: equine.
Purity :
Purified by Protein G affinity chromatography.
Form :
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138592-AF.
Concentration :
~0.2mg/ml
Desc2 :
Product Type: Mab Isotype: IgG2b,k Clone No: 66.4.C2, PN-15 Host: mouse Source: equine Concentration: ~0.2mg/ml Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138592AF. Purity: Purified by Protein G affinity chromatography. Immunogen: Microsomal fraction of human renal cortical tissue homogenate Specificity: Recognizes human Renal Cell Carcinoma. Species Crossreactivity: equine. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
E FC IF IHC IP WB
Storage Temperature :
-20°C
Reference :
1. Yoshida SO, et. al. Cancer Research, 1989, 49(7):1802-9.
Picture 1 File Name :
https://usbio-images.r.worldssl.net/prodimages/16/138592_1.jpg
company information
US Biological
4 Technology Way
Salem, MA01970
service@usbio.net
https://www.usbio.net
800-520-3011
headquarters: USA
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