p40 (p63 Delta) | US Biological 138580 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

p40 (p63 Delta)

catalog :

138580

quantity :

20 ug

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

human

product information

Catalog Number :

138580

Product wo Prefix :

p40 (p63 Delta)

Host :

rabbit

Product Type :

Pab

Category :

Antibodies

Size1 :

20 ug

Isotype :

IgG

Desc1 :

p40 (p63 delta) is a marker recently determined to be highly specific for squamous basal cells in the immunohistochemistry (IHC) application. The current more routinely recommended marker, p63, appears to have less specificity compared to p40, especially on squamous cell tumors. The ability to differentiate between lung adenocarcinoma vs. squamous cell carcinoma is difficult and has bearing on the different therapeutic avenues for each subtype treatment. p63 antibody's ability to distinguish between the tumor types appears to be inferior when compared to p40. The ability to utilize an antibody probe for p40 as a squamous cell marker bolsters its use for future sub-classification of lung cancers, especially by immunohistochemical techniques. Applications: Suitable for use in Flow Cytometry, Western Blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml Flow Cytometry: 0.5-1ug/10e6 cells Immunofluorescence: 1-2ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 1-2ug/ml/30min @ RT Immunoprecipitation: 1-2ug/500ug protein lysate Optimal dilutions to be determined by the researcher. Positive Control: HEK293 cells or Prostate Carcinoma or Lung Squamous Cell Carcinoma Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Bo Hu Mo Rt

Immunogen :

Synthetic peptide (ENNAQTQFSEPQY) corresponding to aa5-17 from human p40.

Specificity :

Recognizes human p40. Species Crossreactivity: mouse, rat and bovine.

Purity :

Purified by Protein G affinity chromatography.

Form :

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138580-AF.

Concentration :

~0.2mg/ml

Desc2 :

Product Type: Pab Isotype: IgG Host: rabbit Source: human Concentration: ~0.2mg/ml Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138580AF. Purity: Purified by Protein G affinity chromatography. Immunogen: Synthetic peptide (ENNAQTQFSEPQY) corresponding to aa5-17 from human p40. Specificity: Recognizes human p40. Species Crossreactivity: mouse, rat and bovine. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

FC IF IHC IP WB

Storage Temperature :

-20°C

Reference :

1. Bishop, JA et. al. Modern Pathology 25: 405-415. 2. Scagliotti G et. al. J Thorac Oncol 6:64-70. 3. Kargi A et. al. Appl Immunohistochem Mol Morphol 15:415-420.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/49/138580_1jpeg