p21 (Cyclin-dependent Kinase Inhibitor, CDK-interacting Protein 1, WAF1)

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

p21 (Cyclin-dependent Kinase Inhibitor, CDK-interacting Protein 1, WAF1)

catalog :

138579

quantity :

20 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

WA-1 (HJ21)

reactivity :

human

product information

Catalog Number :

138579

Product wo Prefix :

p21 (Cyclin-dependent Kinase Inhibitor, CDK-interacting Protein 1, WAF1)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

20 ug

Clone # USB :

WA-1 (HJ21)

Isotype :

IgG1,k

Desc1 :

This monoclonal antibody recognizes a 21kD protein, identified as the p21WAF1 tumor suppressor protein. This monoclonal antibody is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdk's and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma). Applications: Suitable for use in ELISA, Flow Cytometry, Western Blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug/10e6 cells Immunofluorescence: 1-2ug/ml Western Blot: 0.5-1ug/ml Immunoprecipitation: 1-2ug/500ug protein lysate Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT Optimal dilutions to be determined by the researcher. Positive Control: HeLa Cells. Skin, colon, or breast carcinoma. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mk Mo Rt

Immunogen :

Recombinant protein corresponding to human p21.

Specificity :

Recognizes human p21. Species Crossreactivity: monkey, mouse and rat. Does not cross-react with other closely related mitotic inhibitors.

Purity :

Purified by Protein G affinity chromatography.

Form :

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138579.

Concentration :

~0.2mg/ml

Desc2 :

Product Type: Mab Isotype: IgG1,k Clone No: WA-1 (HJ21) Host: mouse Source: human Concentration: ~0.2mg/ml Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138579. Purity: Purified by Protein G affinity chromatography. Immunogen: Recombinant protein corresponding to human p21. Specificity: Recognizes human p21. Species Crossreactivity: monkey, mouse and rat. Does not cross-react with other closely related mitotic inhibitors. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E FC IF IHC IP WB

Storage Temperature :

-20°C

Reference :

1. Krzywicka-Racka A & Sluder G J Cell Biol 194:199-207 (2011). 2. Folini M et al. Biochem Pharmacol 79:1781-90 (2010).

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/48/138579_1.jpg