Neurofilament (NF, NF-H) | US Biological 138577 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Neurofilament (NF, NF-H)

catalog :

138577

quantity :

20 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

NF421

The same clone is also sold as:

Abnova: MAB11233
Novus Biologicals: NBP2-29629, NBP2-33102, NBP2-44724

reactivity :

human

product information

Catalog Number :

138577

Product wo Prefix :

Neurofilament (NF, NF-H)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

20 ug

Clone # USB :

NF421

Isotype :

IgG1

Desc1 :

This monoclonal antibody reacts with a 200kD protein, identified as heavy sub-unit of neurofilaments (NF-H). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kD (NF-L), 160kD (NF-M) and 200kD (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament. Applications: Suitable for use in ELISA, Western Blot, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug/10e6 cells Western Blot: 0.5-1ug/ml Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT Optimal dilutions to be determined by the researcher. Positive Control: Brain, Neuroblastoma Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Ch Hu Mo Po Rt

Immunogen :

Recombinant protein corresponding to human neurofilament 200kD.

Specificity :

Recognizes neurofilament. Species Crossreactivity: human, rat, mouse, porcine and chicken.

Purity :

Purified by Protein G affinity chromatography.

Form :

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138577-AF.

Concentration :

~0.2mg/ml

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: NF421 Host: mouse Source: human Concentration: ~0.2mg/ml Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138577AF. Purity: Purified by Protein G affinity chromatography. Immunogen: Recombinant protein corresponding to human neurofilament 200kD. Specificity: Recognizes neurofilament. Species Crossreactivity: human, rat, mouse, porcine and chicken. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E FC IHC WB

Storage Temperature :

-20°C

Reference :

1. Shaw G et. al. European Journal of Cell Biology. 42:1-9 (1986). 2. Shaw G et. al. European Journal of Cell Biology. 34:130-136 (1984).

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/36/138577_1.jpg