Neurofilament (NF 200kD, NF-200, NF-H, NF) | US Biological 138576 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Neurofilament (NF 200kD, NF-200, NF-H, NF)

catalog :

138576

quantity :

20 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

RT97

The same clone is also sold as:

Developmental Studies Hybridoma Bank: RT97
Abnova: MAB11229
Novus Biologicals: NBP2-29435, NBP2-33062

reactivity :

human

product information

Catalog Number :

138576

Product wo Prefix :

Neurofilament (NF 200kD, NF-200, NF-H, NF)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

20 ug

Clone # USB :

RT97

Isotype :

IgG1

Desc1 :

138576 reacts with a 200kD protein, identified as heavy sub-unit of neurofilaments (NF-H). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kD (NF-L), 160kD (NF-M) and 200kD (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament. Applications: Suitable for use in ELISA, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug/10e6 cells Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissues sections in 10mM sodium citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Optimal dilutions to be determined by the researcher. Positive Control: Brain, Neuroblastoma Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Ch Hu Mo Po Rt

Immunogen :

Triton-X 100 insoluble protein fraction of rat brain

Specificity :

Recognizes rat neurofilament. Species Crossreactivity: human, mouse, chicken and porcine.

Purity :

Purified by Protein G affinity chromatography.

Form :

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138576-AF.

Concentration :

~0.2mg/ml

Desc2 :

Product Type: Mab Isotype: IgG1 Clone No: RT97 Host: mouse Source: rat Concentration: ~0.2mg/ml Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138576-AF. Purity: Purified by Protein G affinity chromatography. Immunogen: Triton-X 100 insoluble protein fraction of rat brain Specificity: Recognizes rat neurofilament. Species Crossreactivity: human, mouse, chicken and porcine. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E FC IHC

Storage Temperature :

-20°C

Reference :

1. Ishii M et. al. Fukushima J Med Sci. 2004;50(2):65-74.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/30/138576_1.jpg