This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
US Biological
product type :
antibody
product name :
Myogenin
catalog :
138574
quantity :
20 ug
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
MGN185

The same clone is also sold as:
reactivity :
human
product information
Catalog Number :
138574
Product wo Prefix :
Myogenin
Host :
mouse
Product Type :
Mab
Category :
Antibodies
Size1 :
20 ug
Clone # USB :
MGN185
Isotype :
IgG1,k
Desc1 :
Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70aa in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80% aa homology in their bHLH motifs. Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms' tumor. Applications: Suitable for use in ELISA, Western Blot, Flow Cytometry, Immunofluorescence, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug/10e6 cells Immunofluorescence: 1-2ug/ml Western Blot: 0.5-1ug/ml Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT Immunoprecipitation: 1-2ug/500ug protein lysate Optimal dilutions to be determined by the researcher. Positive Control: Rh-30 cells. Skeletal muscle or rhabdomyosarcoma Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Calc Crossreactivity :
Fe Hu Mo Po Rt
Immunogen :
Recombinant protein corresponding to human myogenin.
Specificity :
Recognizes human myogenin. Species Crossreactivity: mouse, rat, feline and porcine.
Purity :
Purified by Protein G affinity chromatography.
Form :
Supplied as a liquid in PBS with 0.05% BSA and 0.05% sodium azide. Also available without BSA and azide, see 138574-AF.
Concentration :
~0.2mg/ml
Desc2 :
Product Type: Mab Isotype: IgG1,k Clone No: MGN185 Host: mouse Source: human Concentration: ~0.2mg/ml Form: Supplied as a liquid in PBS with 0.05% BSA and 0.05% sodium azide. Also available without BSA and azide, see 138574AF. Purity: Purified by Protein G affinity chromatography. Immunogen: Recombinant protein corresponding to human myogenin. Specificity: Recognizes human myogenin. Species Crossreactivity: mouse, rat, feline and porcine. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
E FC IF IHC IP WB
Storage Temperature :
-20°C
Reference :
1. Wang NP et. al. Am J Pathol 1995, 147:1799-1810.
Picture 1 File Name :
https://usbio-images.r.worldssl.net/prodimages/18/138574_1.jpg
company information
US Biological
4 Technology Way
Salem, MA01970
service@usbio.net
https://www.usbio.net
800-520-3011
headquarters: USA
United States Biological is a primary brand for the Life Science Industry. We are committed to reducing the cost of research with value, integrity and a truly personal buying experience.