MUC-1 (EMA, Epithelial Membrane Antigen Mucin-1 ) | US Biological 138571 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

MUC-1 (EMA, Epithelial Membrane Antigen Mucin-1 )

catalog :

138571

quantity :

20 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

GP1.4

The same clone is also sold as:

Leica Biosystems: NCL-EMA, PA0039
Invitrogen: MA1-06503
OriGene: AM32842PU-T, AM32842PU-S, TA355293
Novus Biologicals: NBP2-33174, NBP2-29408
Abnova: MAB11313, MAB16236
Abcam: ab134282

reactivity :

human

product information

Catalog Number :

138571

Product wo Prefix :

MUC-1 (EMA, Epithelial Membrane Antigen Mucin-1 )

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

20 ug

Clone # USB :

GP1.4

Isotype :

IgG1,k

Desc1 :

In Western blotting, it recognizes proteins in MW range of 265-400kD, identified as different glycoforms of EMA. This monoclonal antibody reacts with the DTRP epitope in the tandem repeats. The alpha subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling, through phosphorylation s and protein-protein interactions. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinoma tissues. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver. Applications: Suitable for use in ELISA, Flow Cytometry, Western Blot, Immunoprecipitation, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug/10e6 cells Western Blot: 0.5-1ug/ml Immunofluorescence: 1-2ug/ml Immunoprecipitation: 1-2ug/500ug protein lysate Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT Optimal dilutions to be determined by the researcher. Positive Control: MCF-7 or MDA-231 cells. Breast or colon carcinoma. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Immunogen :

Human milk fat globule membrane

Specificity :

Recognizes human EMA.

Purity :

Purified by Protein G affinity chromatography.

Form :

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138571-AF.

Concentration :

~0.2mg/ml

Desc2 :

Product Type: Mab Isotype: IgG1,k Clone No: GP1.4 Host: mouse Source: human Concentration: ~0.2mg/ml Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138571AF. Purity: Purified by Protein G affinity chromatography. Immunogen: Human milk fat globule membrane Specificity: Recognizes human EMA. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E FC IF IHC IP WB

Storage Temperature :

-20°C

Reference :

1. Stanley CM, Phillips TE. Am J Physiol. 1999 Jul;277 (1 Pt 1):G191-200.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/0/138571_1jpeg