This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
US Biological
product type :
antibody
product name :
Melanoma, gp100 (pMel17, Melanocyte Protein PMEL, Melanocytes Lineage-specific Antigen GP100) discontinued
catalog :
138570
quantity :
100 ul
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
HMB45

The same clone is also sold as:
reactivity :
human
product information
Catalog Number :
138570
Product wo Prefix :
Melanoma, gp100 (pMel17, Melanocyte Protein PMEL, Melanocytes Lineage-specific Antigen GP100) discontinued
Host :
mouse
Product Type :
Mab
Category :
Antibodies
Size1 :
100 ul
Clone # USB :
HMB45
Isotype :
IgG1,k
Desc1 :
By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This monoclonal antibody reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, and sarcomas using H & E stains alone. It is also difficult to differentiate melanoma from spindle cell carcinomas and various types of mesenchymal neoplasms. Anti-HMB-45 stains fetal and neonatal melanocytes, junctional and blue nevus cells, and malignant melanoma. This monoclonal antibody also stains Angiomyolipoma (PEComa). Applications: Suitable for use in Flow Cytometry, Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5-10ul/10e6 cells Immunofluorescence: 1:50-1:100 Western Blot: 1:100-1:200 Immunoprecipitation: 5-10ul/500ug protein lysate Immunohistochemistry (Frozen, Formalin fixed paraffin embedded): 1:100-1:200/30min @ RT Optimal dilutions to be determined by the researcher. Positive Control: SK-MEL-28 cells or Melanoma Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Calc Crossreactivity :
Hu
Immunogen :
Extract of pigmented melanoma metastases from lymph nodes
Specificity :
Recognizes human melanoma. Does not react with canine and rat.
Purity :
Supernatant
Form :
Supplied as a liquid, 0.05% sodium azide.
Concentration :
Not determined
Desc2 :
Product Type: Mab Isotype: IgG1,k Clone No: HMB45 Host: mouse Source: human Concentration: Not determined Form: Supplied as a liquid, 0.05% sodium azide. Purity: Supernatant Immunogen: Extract of pigmented melanoma metastases from lymph nodes Specificity: Recognizes human melanoma. Does not react with canine and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
FC IF IHC IP WB
Storage Temperature :
-20°C
Reference :
1. Esclamado RM, et. al. American Journal of Surgery, 1986, 152(4):376-85. 2. Gown AM, et. al. American Journal of Pathology, 1986, 123(2):195-203.
Picture 1 File Name :
https://usbio-images.r.worldssl.net/prodimages/44/138570_1.jpg
company information
US Biological
4 Technology Way
Salem, MA01970
service@usbio.net
https://www.usbio.net
800-520-3011
headquarters: USA
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