Melanoma, gp100 (pMel17, Melanocyte Protein PMEL, Melanocytes Lineage-specific Antigen GP100)

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Melanoma, gp100 (pMel17, Melanocyte Protein PMEL, Melanocytes Lineage-specific Antigen GP100)

catalog :

138569

quantity :

20 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

NKI-beteb

The same clone is also sold as:

Abnova: MAB11274

reactivity :

human

product information

Catalog Number :

138569

Product wo Prefix :

Melanoma, gp100 (pMel17, Melanocyte Protein PMEL, Melanocytes Lineage-specific Antigen GP100)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

20 ug

Clone # USB :

NKI-beteb

Isotype :

IgG2b,k

Desc1 :

By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation. Applications: Suitable for use in ELISA, Flow Cytometry, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug/10e6 cells Immunofluorescence: 1-2ug/ml Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 1-2ug/ml/30min @ RT Optimal dilutions to be determined by the researcher. Positive Control: SK-MEL-28 cells or Melanoma Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Eq Hu

Immunogen :

Membranes from a human melanoma metastasis

Specificity :

Recognizes human melanoma. Species Crossreactivity: equine. Does not react with rat.

Purity :

Purified by Protein G affinity chromatography.

Form :

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138569-AF.

Concentration :

~0.2mg/ml

Desc2 :

Product Type: Mab Isotype: IgG2b,k Clone No: NKI-beteb Host: mouse Source: human Concentration: ~0.2mg/ml Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138569AF. Purity: Purified by Protein G affinity chromatography. Immunogen: Membranes from a human melanoma metastasis Specificity: Recognizes human melanoma. Species Crossreactivity: equine. Does not react with rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E FC IF IHC

Storage Temperature :

-20°C

Reference :

1. Leonhardt RM et al. Proprotein convertases process Pmel17 during secretion. J Biol Chem 286:9321-37 (2011). Flow Cyt ; Human. 2. Leonhardt RM et al. Endoplasmic reticulum export, subcellular distribution, and fibril formation by Pmel17 require an intact N-terminal domain junction. J Biol Chem 285:16166-83 (2010).

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/43/138569_1.jpg