This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
US Biological
product type :
antibody
product name :
MART-1 (Melanoma Antigen Recognize by T cells 1, Melan-A)
catalog :
138568
quantity :
20 ug
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
M2-7C10

The same clone is also sold as:
reactivity :
human
product information
Catalog Number :
138568
Product wo Prefix :
MART-1 (Melanoma Antigen Recognize by T cells 1, Melan-A)
Host :
mouse
Product Type :
Mab
Category :
Antibodies
Size1 :
20 ug
Clone # USB :
M2-7C10
Isotype :
IgG2b,k
Desc1 :
This antibody recognizes a protein doublet of 20-22kD, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is pres ent in melanosomes and endoplasmic reticulum. This monoclonal antibody labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Applications: Suitable for use in ELISA, Immunofluorescence, Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug/10e6 cells Western Blot: 0.5-1ug/ml Immunofluorescence: 1-2ug/ml Immunoprecipitation: 1-2ug/500ug protein lysate Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT Optimal dilutions to be determined by the researcher. Positive Control: SK-MEL-13 and SK-MEL-19 Melanoma cell lines; Melanomas Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Calc Crossreactivity :
Hu
Immunogen :
Recombinant protein corresponding to human MART-1.
Specificity :
Recognizes human MART-1. Does not react with mouse or rat.
Purity :
Purified by Protein G affinity chromatography.
Form :
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138568-AF.
Concentration :
~0.2mg/ml
Desc2 :
Product Type: Mab Isotype: IgG2b,k Clone No: M2-7C10 Host: mouse Source: human Concentration: ~0.2mg/ml Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138568AF. Purity: Purified by Protein G affinity chromatography. Immunogen: Recombinant protein corresponding to human MART-1. Specificity: Recognizes human MART-1. Does not react with mouse or rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
E FC IF IHC IP WB
Storage Temperature :
-20°C
Reference :
1. Kawakami Y, et. al. Journal of Immunological Methods, 1997, 202(1):13-25. 2. Marincola FM, et. al. Journal of Immunotherapy with Emphasis on Tumor I mmunology, 1996, 19(3):192-205.
Picture 1 File Name :
https://usbio-images.r.worldssl.net/prodimages/37/138568_1.jpg
company information
US Biological
4 Technology Way
Salem, MA01970
service@usbio.net
https://www.usbio.net
800-520-3011
headquarters: USA
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