This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
US Biological
product type :
antibody
product name :
MAGE-1 (Melanoma-associated Antigen 1, MAGE-1 Antigen, Cancer/testis Antigen, CTA)
catalog :
138566
quantity :
20 ug
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
MA454

The same clone is also sold as:
reactivity :
human
product information
Catalog Number :
138566
Product wo Prefix :
MAGE-1 (Melanoma-associated Antigen 1, MAGE-1 Antigen, Cancer/testis Antigen, CTA)
Host :
mouse
Product Type :
Mab
Category :
Antibodies
Size1 :
20 ug
Clone # USB :
MA454
Isotype :
IgG1,k
Desc1 :
Recognizes a protein of 42-46kD, identified as MAGE-1. This monoclonal antibody does not cross-react with MAGE- 2, -3, -4, -6 -9, -10, -or -12 protein. Human malignant neoplasms carry rejection antigens that are recognized by the patients' autologous, tumor directed and specific, cytolytic, CD8+ T lymphocyte clones (CTL). The MAGE family of genes codes an important group of antigens. It was identified that melanomas and primary glial brain tumors express common melanoma associated antigens (MAAs). Because MAGE-1 is expressed on a significant proportion of human neoplasms of various histological types (melanoma, brain tumors of glial origin, neurobla stoma, non-small cell lung cancer, breast, gastric, colorectal, ovarian, renal cell carcinomas) and not on normal tissues, the encoded antigen may serve as a marker of early detection and target for cancer immunotherapy. Applications: Suitable for use in ELISA, Western Blot, Flow Cytometry, Immunofluorescence, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml Flow Cytometry: 0.5-1ug/10e6 cells Immunofluorescence: 1-2ug/ml Immunoprecipitation: 1-2ug/500ug protein lysate Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT Optimal dilutions to be determined by the researcher. Positive Control: Melanoma cell lines. Melanomas, gliomas, neuroblastoma, non-small cell lung cancer, breast, gastric, colorectal, ovarian, and renal cell carcinomas. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Calc Crossreactivity :
Ca Hu Rt
Immunogen :
Recombinant protein corresponding to full length human MAGE-1.
Specificity :
Recognizes human MAGE-1. Species Crossreactivity: rat and canine.
Purity :
Purified by Protein G affinity chromatography.
Form :
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138566-AF.
Concentration :
~0.2mg/ml
Desc2 :
Product Type: Mab Isotype: IgG1,k Clone No: MB454 Host: mouse Source: human Concentration: ~0.2mg/ml Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138566AF. Purity: Purified by Protein G affinity chromatography. Immunogen: Recombinant protein corresponding to full length human MAGE-1. Specificity: Recognizes human MAGE-1. Species Crossreactivity: rat and canine. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
E FC IF IHC IP WB
Storage Temperature :
-20°C
Reference :
1. Ma ZY, Khatlani TS, Sasaki K, Okuda M, Inokuma H, Onishi T. Identification of feline MAGE-1 gene product by monoclonal antibodies. Tissue Antigens. 2002 Aug;60(2):133-8. PMID: 12392507 2. Laduron S, Deplus R, Zhou S, Kholmanskikh O, Godelaine D, De Smet C, Hayward SD, Fuks F, Boon T, De Plaen E. MAGE-A1 interacts with adaptor SKIP and the deacetylase HDAC1 to repress transcription. Nucleic Acids Res. 2004 Aug 17;32(14):4340-50. Print 2004. PMID: 15316101 3. Loriot A, De Plaen E, Boon T, De Smet C. Transient down-regulation of DNMT1 methyltransferase leads to activation and stable hypomethylation of MAGE-A1 in melanoma cells. J Biol Chem. 2006 Apr 14;281(15):10118-26. Epub 2006 Feb 22. PMID: 16497664 4. Pereira CM, Gomes CC, De Fátima Correia Silva J, Bucciarelli Rodriguez M, Barbosa AA, Gomez RS. Evaluation of MAGE A1 in oral squamous cell carcinoma. Oncol Rep. 2012 Jun;27(6):1843-8. doi: 10.3892/or.2012.1736. Epub 2012 Mar 19. PMID: 22447174
Picture 1 File Name :
https://usbio-images.r.worldssl.net/prodimages/25/138566_1.jpg
company information
US Biological
4 Technology Way
Salem, MA01970
service@usbio.net
https://www.usbio.net
800-520-3011
headquarters: USA
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