Keratin, HMW (High Molecular Weight CytoKeratin) discontinued | US Biological 138562 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Keratin, HMW (High Molecular Weight CytoKeratin) discontinued

catalog :

138562

quantity :

100 ul

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

34bE12

The same clone is also sold as:

Abnova: MAB11309
Abcam: ab776

reactivity :

human

product information

Catalog Number :

138562

Product wo Prefix :

Keratin, HMW (High Molecular Weight CytoKeratin) discontinued

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

100 ul

Clone # USB :

34bE12

Isotype :

IgG1,k

Desc1 :

This antibody recognizes CK1, CK5, CK10 and CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This monoclonal antibody shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands; there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations. Applications: Suitable for use in Flow Cytometry, Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5-10ul/10e6 cells Immunofluorescence: 1:50-1:100 Western Blot: 1:100-1:200 Immunoprecipitation: 5-10ul/500ug protein lysate Immunohistochemistry (Formalin fixed paraffin embedded): 1:100-1:200/30min @ RT Optimal dilutions to be determined by the researcher. Positive Control: PC12 cells, Skin, Prostate carcinoma Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Hu Mo Rt

Immunogen :

Solublized keratin extract from human stratum corneum

Specificity :

Recognizes human cytokeratin. Species Crossreactivity: mouse and rat. Reacts with squamous, ductal and other complex epithelia. It stains adenocarcinomas, breast, pancreas, bile duct, salivary gland and transitional cell carcinomas.

Purity :

Supernatant

Form :

Supplied as a liquid in 0.05% sodium azide.

Concentration :

Not determined

Desc2 :

Product Type: Mab Isotype: IgG1,k Clone No: 34bE12 Host: mouse Source: human Concentration: Not determined Form: Supplied as a liquid in 0.05% sodium azide. Purity: Supernatant Immunogen: Solublized keratin extract from human stratum corneum Specificity: Recognizes human cytokeratin. Species Crossreactivity: mouse and rat. Reacts with squamous, ductal and other complex epithelia. It stains adenocarcinomas, breast, pancreas, bile duct, salivary gland and transitional cell carcinomas. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

FC IF IHC IP WB

Storage Temperature :

-20°C

Reference :

1. Moinfar F et. al. Am J Surg Pathol 1999;23(9):1048-58. 2. Varma M et. al. Mod Pathol 1999;12(5):472-8.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/1/138562_1.jpg