Keratin 19 (K19, Keratin-19, CK-19, Cytokeratin-19) | US Biological 138560 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Keratin 19 (K19, Keratin-19, CK-19, Cytokeratin-19)

catalog :

138560

quantity :

20 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A53-B/A2.26, Ks 19.1

reactivity :

human

product information

Catalog Number :

138560

Product wo Prefix :

Keratin 19 (K19, Keratin-19, CK-19, Cytokeratin-19)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

20 ug

Clone # USB :

A53-B/A2.26, Ks 19.1

Isotype :

IgG2a,k

Desc1 :

This monoclonal antibody reacts with the rod domain of human cytokeratin-19 (CK19), a polypeptide of 40kD. Its epitope maps between aa312-335. CK19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer. Applications: Suitable for use in ELISA, Western Blot, Flow Cytometry, Immunoprecipitation, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml Flow Cytometry: 0.5-1ug/10e6 cells Immunofluorescence: 1-2ug/ml Immunoprecipitation: 1-2ug/500ug protein lysate Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT Optimal dilutions to be determined by the researcher. Positive Control: MCF-7, HeLa, Hep-G2 cells, Breast Cancer Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Immunogen :

Human breast cancer MCF-7 cells

Specificity :

Recognizes human keratin 19. Weakly binds to mouse, rat and guinea pig Keratin-19.

Purity :

Purified by Protein G affinity chromatography.

Form :

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138560.

Concentration :

~0.2mg/ml

Desc2 :

Product Type: Mab Isotype: IgG2a,k Clone No: A53-B/A2.26, Ks 19.1 Host: mouse Source: human Concentration: ~0.2mg/ml Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138560. Purity: Purified by Protein G affinity chromatography. Immunogen: Human breast cancer MCF-7 cells Specificity: Recognizes human keratin 19. Weakly binds to mouse, rat and guinea pig Keratin-19. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E FC IF IHC IP WB

Storage Temperature :

-20°C

Reference :

1. Bartek J et. al. Histochemical Journal, 1990, 22(10):537-44.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/39/138560_1jpeg