This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
US Biological
product type :
antibody
product name :
Insulin
catalog :
138556
quantity :
20 ug
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
2D11-H5

The same clone is also sold as:
reactivity :
human
product information
Catalog Number :
138556
Product wo Prefix :
Insulin
Host :
mouse
Product Type :
Mab
Category :
Antibodies
Size1 :
20 ug
Clone # USB :
2D11-H5
Isotype :
IgG1,k
Desc1 :
Recognizes a polypeptide which is identified as insulin, a 51aa polypeptide composed of A and B chains connected through the C-peptide. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-terminal basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain ions. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. Deficiency of insulin results in diabetes mellitus. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as beta-cell and insulinoma marker. Applications: Suitable for use in Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 0.5-1ug/10e6 cells Immunofluorescence: 1-2ug/ml Immunohistochemistry (FFPE): 0.1-0.2ug/ml for 30 minutes at RT No special treatment is required for staining of formalin/paraffin tissues. Optimal dilutions to be determined by the researcher. Positive Control: MIA PaCa-2 cells or Pancreas Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Calc Crossreactivity :
Bo Hu Mo Po
Immunogen :
Purified porcine insulin, conjugatd to KLH. Cellular Localization: Cytoplasmic
Specificity :
Recognizes porcine Insulin. Species Crossreactivity: human, bovine and mouse
Purity :
Purified by Protein G affinity chromatography.
Form :
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138556-AF.
Concentration :
~0.2mg/ml
Desc2 :
Product Type: Mab Isotype: IgG1,k Clone No: 2D11-H5 Host: mouse Source: porcine Concentration: ~0.2mg/ml Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138556-AF. Purity: Purified by Protein G affinity chromatography. Immunogen: Purified porcine insulin, conjugatd to KLH. Cellular Localization: Cytoplasmic Specificity: Recognizes porcine Insulin. Species Crossreactivity: human, bovine and mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
FC IF IHC
Storage Temperature :
-20°C
Reference :
1. de la Tour, D., et al. 2001. Mol. Endoc. 15: 476-483. 2. Rajagopal, J., et al. 2003. Science 299: 363. 3. Morisset, J., et al. 2003. J. Histochem. Cytochem. 51: 1501-1513.
Picture 1 File Name :
https://usbio-images.r.worldssl.net/prodimages/20/138556_1.jpg
company information
US Biological
4 Technology Way
Salem, MA01970
service@usbio.net
https://www.usbio.net
800-520-3011
headquarters: USA
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