This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
US Biological
product type :
antibody
product name :
Glial Fibrillary Acidic Protein (GFAP)
catalog :
138553
quantity :
20 ug
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
GA-5

The same clone is also sold as:
reactivity :
human
product information
Catalog Number :
138553
Product wo Prefix :
Glial Fibrillary Acidic Protein (GFAP)
Host :
mouse
Product Type :
Mab
Category :
Antibodies
Size1 :
20 ug
Clone # USB :
GA-5
Isotype :
IgG1
Desc1 :
This monoclonal antibody recognizes a protein of ~50kD which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS. Applications: Suitable for use in ELISA, Western Blot, Flow Cytometry, Immunofluorescence, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml Flow Cytometry: 0.5-1ug/10e6 cells Immunofluorescence: 1-2ug/ml Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT Immunoprecipitation: 1-2ug/500ug protein lysate Optimal dilutions to be determined by the researcher. Positive Control: Brain or Astrocytoma Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Calc Crossreactivity :
Bo Ch Hu Mo Po Rb Rt
Immunogen :
GFAP isolated from pig spinal cord
Specificity :
Recognizes GFAP. Species Crossreactivity: human, mouse, rat, bovine, porcine, rabbit and chicken.
Purity :
Purified by Protein G affinity chromatography.
Form :
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138553-AF.
Concentration :
~0.2mg/ml
Desc2 :
Product Type: Mab Isotype: IgG1 Clone No: GA-5 Host: mouse Source: human Concentration: ~0.2mg/ml Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138553-AF. Purity: Purified by Protein G affinity chromatography. Immunogen: GFAP isolated from pig spinal cord Specificity: Recognizes GFAP. Species Crossreactivity: human, mouse, rat, bovine, porcine, rabbit and chicken. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
E FC IF IHC IP WB
Storage Temperature :
-20°C
Reference :
1. Herpers MJ et. Al. 1986, Acta Neuropathol, 70:333-339. 2. Van Muijen GN et. al. 1987, Lab Invest, 57:359-369. 3. Debus E, et. al. 1983, Differentiation, 25(2):193-203.
Picture 1 File Name :
https://usbio-images.r.worldssl.net/prodimages/2/138553_1jpeg
company information
US Biological
4 Technology Way
Salem, MA01970
service@usbio.net
https://www.usbio.net
800-520-3011
headquarters: USA
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