Estrogen Receptor, beta (ES) | US Biological 138550 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Estrogen Receptor, beta (ES)

catalog :

138550

quantity :

20 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

ERb455

The same clone is also sold as:

Abnova: MAB11240, MAB14209
Novus Biologicals: NBP2-44365, NBP2-33066, NBP2-29439
Abcam: ab187291

reactivity :

human

product information

Catalog Number :

138550

Product wo Prefix :

Estrogen Receptor, beta (ES)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

20 ug

Clone # USB :

ERb455

Isotype :

IgG2a

Desc1 :

Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ER-alpha and ER-beta, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ER-alpha and ER-beta are differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ER-alpha and ER-beta may be regulated by distinct mechanisms even though they share many functional characteristics. Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence: 1-2ug/ml Western Blot: 0.5-1ug/ml Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT Immunoprecipitation: 1-2ug/500ug protein lysate Optimal dilutions to be determined by the researcher. Positive Control: Ovarian, breast or prostate carcinoma Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Eq Hu Mk Mo Po Rt Sh

Immunogen :

Recombinant protein corresponding to human Estrogen Receptor beta at C-terminus.

Specificity :

Recognizes human Estrogen Receptor beta. Species Crossreactivity: monkey, mouse, rat, porcine, equine and sheep.

Purity :

Purified by Protein G affinity chromatography.

Form :

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138550-AF.

Concentration :

~0.2mg/ml

Desc2 :

Product Type: Mab Isotype: IgG2a Clone No: ERb455 Host: mouse Source: human Concentration: ~0.2mg/ml Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138550AF. Purity: Purified by Protein G affinity chromatography. Immunogen: Recombinant protein corresponding to human Estrogen Receptor beta at C-terminus. Specificity: Recognizes human Estrogen Receptor beta. Species Crossreactivity: monkey, mouse, rat, porcine, equine and sheep. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E IF IHC IP WB

Storage Temperature :

-20°C

Reference :

1. Skliris GP et. al. J Pathol 2002;197:155-62.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/34/138550_1.jpg