This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
US Biological
product type :
antibody
product name :
Epithelial Membrane Antigen (MUC-1, Mucin-1, CA15-3, CA27-29, CD227, DF3, EMA, EMA E29, H23AG, KL-6, MAM6, PEM, PEMT, PUM) discontinued
catalog :
138546
quantity :
100 ul
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
E29

The same clone is also sold as:
reactivity :
human
product information
Catalog Number :
138546
Product wo Prefix :
Epithelial Membrane Antigen (MUC-1, Mucin-1, CA15-3, CA27-29, CD227, DF3, EMA, EMA E29, H23AG, KL-6, MAM6, PEM, PEMT, PUM) discontinued
Host :
mouse
Product Type :
Mab
Category :
Antibodies
Size1 :
100 ul
Clone # USB :
E29
Isotype :
IgG2a,k
Desc1 :
In Western blotting, it recognizes proteins in MW range of 265-400kD, identified as different glycoforms of EMA. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit and the C-terminal beta subunit. The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling, through phosphorylations and protein-protein interactions. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinoma tissues. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver. Applications: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5-10ul/10e6 cells Immunofluorescence: 1:50-1:100 Western Blot: 1:100-1:200 Immunoprecipitation: 5-10ul/500ug protein lysate Immunohistochemistry (Formalin fixed paraffin embedded): 1:100-1:200/30min @ RT Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Calc Crossreactivity :
Hu
Immunogen :
Delipidated extract of human milk fat globule membranes
Specificity :
Recognizes human EMA.
Purity :
Supernatant
Form :
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Concentration :
Not determined
Desc2 :
Product Type: Mab Isotype: IgG2a,k Clone No: E29 Host: mouse Source: human Concentration: Not determined Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Purity: Supernatant Immunogen: Delipidated extract of human milk fat globule membranes Specificity: Recognizes human EMA. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
FC IF IHC IP WB
Storage Temperature :
-20°C
Reference :
1. Cordell J et al. 1985. Br J Cancer 52(3):347-54. 2. Heyderman E et al. 1985. Br J Cancer 52(3):355-61.
Picture 1 File Name :
https://usbio-images.r.worldssl.net/prodimages/15/138546_1.jpg
company information
US Biological
4 Technology Way
Salem, MA01970
service@usbio.net
https://www.usbio.net
800-520-3011
headquarters: USA
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