This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
US Biological
product type :
antibody
product name :
EpCAM (CD326, Epithelial Cell Adhesion Molecule,
Epithelial Cell Surface Antigen) discontinued
catalog :
138542
quantity :
100 ul
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
GZ-20
reactivity :
rat
product information
Catalog Number :
138542
Product wo Prefix :
EpCAM (CD326, Epithelial Cell Adhesion Molecule,
Epithelial Cell Surface Antigen) discontinued
Host :
mouse
Product Type :
Mab
Category :
Antibodies
Size1 :
100 ul
Clone # USB :
GZ-20
Isotype :
IgG1
Desc1 :
Epitope of GZ-1 and GZ-20 monoclonal antibodies are different. EGP40 is a 40-43kD transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It i s expressed on baso-lateral cell surface in most simple epithelia and a vast majority of ca cinomas.
Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1:100-1:200
Immunohistochemistry (Formalin fixed paraffin embedded): 1:50-1:100/30min @ RT
Flow Cytometry: 5-10ul/10e6 cells
Immunofluorescence: 1:50-1:100
Immunoprecipitation: 5-10ul/500ug protein lysate
Optimal dilutions to be determined by the researcher.
Positive Control:
Rat small intestine or colon
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Calc Crossreactivity :
Rt
Immunogen :
Rat small intestine cells
Specificity :
Rat small intestine cells
Purity :
Supernatant
Form :
Supplied as a liquid in 0.05% sodium azide.
Concentration :
Not determined
Desc2 :
Product Type: Mab
Isotype: IgG1
Clone No: GZ-20
Host: mouse
Source: rat
Concentration: Not determined
Form: Supplied as a liquid in 0.05% sodium azide.
Purity: Supernatant
Immunogen: Rat small intestine cells
Specificity: Recognizes rat EpCAM. Does not recognize human.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
FC IF IHC IP WB
Storage Temperature :
-20°C
Reference :
1. Litvinov SV et. al. J Cell Biol, 1994, 125:437-46. 2. Tsubura A et. al. J Cut Pathol, 1992, 19:73-9.
Picture 1 File Name :
https://usbio-images.r.worldssl.net/prodimages/41/138542_1jpeg
company information
US Biological
4 Technology Way
Salem, MA01970
Salem, MA01970
service@usbio.net
https://www.usbio.net800-520-3011
headquarters: USA
United States Biological is a primary brand for the Life Science Industry. We are committed to reducing the cost of research with value, integrity and a truly personal buying experience.
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