Cytokeratin, pan (Pan Keratin) | US Biological 138538 product information

product summary

company name :

US Biological

product type :

antibody

product name :

Cytokeratin, pan (Pan Keratin)

catalog :

138538

quantity :

20 ug

price :

353 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AE-1/AE-3

reactivity :

human

more info or order :

US Biological product webpage

citations: 1

Reference
Wang X, Deavers M, Patenia R, Bassett R, Mueller P, Ma Q, et al. Monocyte/macrophage and T-cell infiltrates in peritoneum of patients with ovarian cancer or benign pelvic disease. J Transl Med. 2006;4:30 pubmed

product information

Catalog Number :

138538

Product wo Prefix :

Cytokeratin, pan (Pan Keratin)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

20 ug

Size1 List :

353 USD

Clone # USB :

AE-1/AE-3

Isotype :

IgG1,k

Desc1 :

Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which include CK1, CK3, CK4, CK5 , CK6, CK8, CK10, CK14, CK15, CK16, and CK19. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE-1/AE-3 is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing ce lls in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas. Applications: Suitable for use in Western Blot, Flow Cytometry, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml for 2 hours at RT Flow Cytometry: 0.5-1ug/10e6 cells Immunofluorescence: 1-2ug/ml Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissues sections in 10mM sodium citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Optimal dilutions to be determined by the researcher. Positive Control: Skin, Adeno- or Squamous carcinomas Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Bo Ca Ch Hu Mk Mo Rb Rt

Immunogen :

Human epidermal keratin

Specificity :

Recognizes human pan cytokeratin. Species Crossreactivity: monkey, bovine, canine, rabbit, mouse, rat and chicken.

Purity :

Purified by Protein G affinity chromatography.

Form :

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA & azide, see 138538-AF.

Concentration :

~0.2mg/ml

Desc2 :

Product Type: Mab Isotype: IgG1,k Clone No: AE-1/AE-3 Host: mouse Source: human Concentration: ~0.2mg/ml Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA & azide, see 138538-AF. Purity: Purified by Protein G affinity chromatography. Immunogen: Human epidermal keratin Specificity: Recognizes human pan cytokeratin. Species Crossreactivity: monkey, bovine, canine, rabbit, mouse, rat and chicken. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

FC IF IHC WB

Storage Temperature :

-20°C

Reference :

1. Woodock-Mitchell J et. al. Journal of Cell Biology 1982;95:580-8. 2. Tseng SCG et. al. Cell 1982; 30361.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/22/138538_1.jpg

more info or order :

US Biological product webpage