Cytokeratin, HMW (High Molecular Weight Keratin) | US Biological 138537 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

Cytokeratin, HMW (High Molecular Weight Keratin)

catalog :

138537

quantity :

20 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

AE-3

The same clone is also sold as:

Novus Biologicals: NBP2-33170, NBP2-29404
Invitrogen: MA1-26281, MA1-5762, MA1-35368, MA5-13150, 14-9000-82, MA1-34220
Abcam: ab16111

reactivity :

human

product information

Catalog Number :

138537

Product wo Prefix :

Cytokeratin, HMW (High Molecular Weight Keratin)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

20 ug

Clone # USB :

AE-3

Isotype :

IgG1,k

Desc1 :

This monoclonal antibody recognizes basic (Type II or HMW) cytokeratins, which include 67kD (CK1); 64kD (CK3); 59kD (CK4); 58kD (CK5); 56kD (CK6); 52kD (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40kD. Monoclonal antibody AE3 recognizes the 65-67, 64, 59, 58, 56, and 52kD keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. Applications: Suitable for use in ELISA, Immunofluorescence, Western Blot, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug/10e6 cells Western Blot: 0.5-1ug/ml Immunofluorescence: 1-2ug/ml Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT Optimal dilutions to be determined by the researcher. Positive Control: Skin, Adenocarcinomas Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Bo Ca Ch Hu Mk Mo Rb Rt

Immunogen :

Human epidermal keratin

Specificity :

Recognizes human cytokeratin. Species Crossreactivity: monkey, bovine, canine, rabbit, mouse, rat and chicken.

Purity :

Purified by Protein G affinity chromatography.

Form :

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138537-AF.

Concentration :

~0.2mg/ml

Desc2 :

Product Type: Mab Isotype: IgG1,k Clone No: AE-3 Host: mouse Source: human Concentration: ~0.2mg/ml Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138537-AF. Purity: Purified by Protein G affinity chromatography. Immunogen: Human epidermal keratin Specificity: Recognizes human cytokeratin. Species Crossreactivity: monkey, bovine, canine, rabbit, mouse, rat and chicken. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E FC IF IHC WB

Storage Temperature :

-20°C

Reference :

1. Woodock-Mitchell J et. al. Journal of Cell Biology 1982;95:580-8. 2. Tseng SCG et. al. Cell 1982; 30361.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/16/138537_1.jpg