This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
US Biological
product type :
antibody
product name :
Cyclin D1
catalog :
138535
quantity :
20 ug
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
DCS-6

The same clone is also sold as:
reactivity :
human
product information
Catalog Number :
138535
Product wo Prefix :
Cyclin D1
Host :
mouse
Product Type :
Mab
Category :
Antibodies
Size1 :
20 ug
Clone # USB :
DCS-6
Isotype :
IgG2a,k
Desc1 :
Recognizes a protein of 36kD, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t(11; 14)(q13; q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1. Applications: Suitable for use in ELISA, Western Blot, Flow Cytometry, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml Flow Cytometry: 0.5-1ug/10e6 cells Immunofluorescence: 1-2ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT Requires HIER using sodium citrate buffer pH6.0 prior to staining. Optimal dilutions to be determined by the researcher. Positive Control: T47D, ZR75, BT474, SKBR3, MCF-7, MDA-MB-453, HT29, Ramos, Jurkat or A431 cells. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Calc Crossreactivity :
Hu Mk Mo Rt
Immunogen :
Recombinant protein corresponding to full length human cyclin D1. SwissProt Accession #: P24385.
Specificity :
Recognizes human cyclin D1. Species Crossreactivity: monkey, mouse and rat.
Purity :
Purified by Protein A/G affinity chromatography.
Form :
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138535-AF.
Concentration :
~0.2mg/ml
Desc2 :
Product Type: Mab Isotype: IgG2a,k Clone No: DCS-6 Host: mouse Source: human Concentration: ~0.2mg/ml Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138535-AF. Purity: Purified by Protein A/G affinity chromatography. Immunogen: Recombinant protein corresponding to full length human cyclin D1. SwissProt Accession #: P24385. Specificity: Recognizes human cyclin D1. Species Crossreactivity: monkey, mouse and rat. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
E FC IF IHC WB
Storage Temperature :
-20°C
Reference :
1. Lukas J, et. al. Oncogene, 1994, 9(3):707-18. 2. Gillett C, et. al. Cancer Research, 1994, 54(7):1812-7. 3. Bartkova J, et. al. Journal of Pathology, 1994, 172(3):237-45.
Picture 1 File Name :
https://usbio-images.r.worldssl.net/prodimages/4/138535_1jpeg
company information
US Biological
4 Technology Way
Salem, MA01970
service@usbio.net
https://www.usbio.net
800-520-3011
headquarters: USA
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