This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
US Biological
product type :
antibody
product name :
CD79a (B Cell Antigen Receptor Complex-associated Protein Alpha Chain) discontinued
catalog :
138528
quantity :
100 ul
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
HM47/A9
The same clone is also sold as:
The same clone is also sold as:
- Invitrogen: MA5-13212
- Abnova: MAB11245
- OriGene: AM00232PU-S, AM00232PU-T
- Novus Biologicals: NBP2-44738-0.1mg, NBP2-34637-0.1mg, NBP2-48028
reactivity :
human
product information
Catalog Number :
138528
Product wo Prefix :
CD79a (B Cell Antigen Receptor Complex-associated Protein Alpha Chain) discontinued
Host :
mouse
Product Type :
Mab
Category :
Antibodies
Size1 :
100 ul
Clone # USB :
HM47/A9
Isotype :
IgG1,k
Desc1 :
A disulphide-linked heterodimer, consisting of mb-1 (or CD79a) and B29 (or CD79b) polypeptides, is non-covalently associated with membrane-bound immunoglobulins on B cells. This complex of mb-1 and B29 polypeptides and immunoglobulin constitute the B cell Ag receptor. CD79a first appears at pre B cell stage, early in maturation, and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in the majority of acute leukemias of precursor B cell type, in B cell lines, B cell lymphomas, and in some myelomas. It is not present in myeloid or T cell lines. Anti-CD79a is generally used to complement anti-CD20 especially for mature B-cell lymphomas after treatment with Rituximab (anti-CD20). This antibody will stain many of the same lymphomas as anti-CD20, but also is more likely to stain B-lymphoblastic lymphoma/leukemia than is anti-CD20. Anti-CD79a also stains more cases of plasma cell myeloma and occasionally some types of endothelial cells as well.
Applications:
Suitable for use in Flow Cytometry, Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 5-10ul/10e6 cells
Immunofluorescence: 1:50-1:100
Western Blot: 1:100-1:200
Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 1:100-1:200/30min @ RT
Immunoprecipitation: 5-10ul/500ug protein lysate
Optimal dilutions to be determined by the researcher.
Positive Control:
Daudi or Ramos cells. Germinal center B-cells in a lymph node or tonsil.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Calc Crossreactivity :
Bo Hu Mk Mo Po Rt
Immunogen :
Recombinant protein fragment corresponding to human CD79a.
Specificity :
Recognizes human CD79a. Species Crossreactivity: mouse, rat, monkey, porcine and bovine.
Purity :
Supernatant
Form :
Supplied as a liquid, 0.05% sodium azide.
Concentration :
Not determined
Desc2 :
Product Type: Mab
Isotype: IgG1,k
Clone No: HM47/A9
Host: mouse
Source: human
Concentration: Not determined
Form: Supplied as a liquid, 0.05% sodium azide.
Purity: Supernatant
Immunogen: Recombinant protein fragment corresponding to human CD79a.
Specificity: Recognizes human CD79a. Species Crossreactivity: mouse, rat, monkey, porcine and bovine.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
FC IF IHC IP WB
Storage Temperature :
-20°C
Reference :
1. Mason, DY, et al. 1995. Blood 86: 1453-1459.
Picture 1 File Name :
https://usbio-images.r.worldssl.net/prodimages/17/138528_1.jpg
company information
US Biological
4 Technology Way
Salem, MA01970
Salem, MA01970
service@usbio.net
https://www.usbio.net800-520-3011
headquarters: USA
United States Biological is a primary brand for the Life Science Industry. We are committed to reducing the cost of research with value, integrity and a truly personal buying experience.
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