antibody

CD56 (Neural Cell Adhesion Molecule, NCAM)

138521

20 ug

monoclonal

mouse

nonconjugated

123C3.D5

The same clone is also sold as:

• Abnova: MAB11301
• Novus Biologicals: NBP2-33132, NBP2-15184-0.1mg
• Abcam: ab270283
• OriGene: TA327645, AM10068SU-N

CD56 (Neural Cell Adhesion Molecule, NCAM)

mouse

Mab

Antibodies

20 ug

123C3.D5

IgG1,k

CD56, a 175-220KD glycoprotein, is a member of the Ig super family. It is expressed as three major isoforms and consists of five Ig-like domains and two Fibronectin-type III domains in the extracellular region. The 135kD isoform is the basic molecule which is glycosylated or sialylated to produce the mature species. CD56 is widely expressed in nervous system, on NK cells and a specific set of Tcells. CD56+ NK cells and Tcells are unique in their ability to mediate cell-mediated cytotoxicity against certain tumor cell targets without MHC restriction. Other physiological functions of CD56 include mediating cell adhesion through homophilic and heterophilic interaction and activating intracellular signaling pathways resulting in neutrite extension and fasciculation, migration and synapses formation in brain. CD56 is also vital for neuronal development and plasticity in adult brain. It is used as a tumor marker in various cancers such as NK lymphomas and Merkel cell carcinoma. NCAM is expressed on most neuroectodermal derived lines, tissues, and neoplasms such as retinoblastoma, medulloblastoma, astrocytoma, and neuroblastoma. It is also expressed on some mesodermally derived tumors such as rhabdomyosarcoma and also on natural killer cells. Applications: Suitable for use in ELISA, Immunofluorescence, Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug/10e6 cells Immunofluorescence: 1-2ug/ml Western Blot: 0.5-1ug/ml Immunoprecipitation: 1-2ug/500ug protein lysate Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT Optimal dilutions to be determined by the researcher. Positive Control: Cerebellum, Pancreas, Neuroblastoma Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Hu Rt Ze

Membrane preparation of a small cell lung carcinoma

Recognizes human CD56. Species Crossreactivity: rat and zebrafish. Recognizes two proteins (185kD & 145kD), identified as two isoforms of neural cell adhesion molecule (NCAM/CD56).

Purified by Protein G affinity chromatography.

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138521-AF.

~0.2mg/ml

Product Type: Mab Isotype: IgG1,k Clone No: 123C3.D5 Host: mouse Source: human Concentration: ~0.2mg/ml Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138521AF. Purity: Purified by Protein G affinity chromatography. Immunogen: Membrane preparation of a small cell lung carcinoma Specificity: Recognizes human CD56. Species Crossreactivity: rat and zebrafish. Recognizes two proteins (185kD & 145kD), identified as two isoforms of neural cell adhesion molecule (NCAM/CD56). Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

E FC IF IHC IP WB

-20°C

1. Gerardy-Schahn R; et. al. Int Journal of Cancer. Supplement, 1994, 8:38-42. 2. Michalides R; et. al. International Journal of Cancer. Supplement, 1994, 8:34-7. 3. Schol DJ; et. al. Int J Cancer. Supplement, 1988, 2:34-40.

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