This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
US Biological
product type :
antibody
product name :
CD45RO (Leukocyte Common Antigen, LCA)
catalog :
138519
quantity :
20 ug
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
UCHL-1

The same clone is also sold as:
reactivity :
human
product information
Catalog Number :
138519
Product wo Prefix :
CD45RO (Leukocyte Common Antigen, LCA)
Host :
mouse
Product Type :
Mab
Category :
Antibodies
Size1 :
20 ug
Clone # USB :
UCHL-1
Isotype :
IgG2a,k
Desc1 :
The CD45RA and CD45RO isoforms of the leukocyte common antigen identify functionally distinct naive and memory T cell subsets. In vitro, CD45RA+ / CD45RO - peripheral blood lymphocytes (PBL) can be converted to CD45RA- / CD45RO + phenotype upon activation in the presence of IL-2. Both CD3+ and CD3-/CD56+ lymphocyte subsets can be converted to CD45RO + lymphocytes. Expression of CD45RO was observed only in response to IL-2 and was not observed during long-term culture in IL-4, IL-6, or IL-7. This antibody recognizes an 180kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. Shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Though this antibody is useful to identify T-cell lymphomas and leukemia, rare staining with B cell lymphomas reported. Applications: Suitable for use in ELISA, Flow Cytometry, Western Blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-2ug/10e6 cells Western Blot: 0.5-1ug/ml Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT Immunofluorescence: 1-2ug/ml Immunoprecipitation: 1-2ug/500ug protein lysate Optimal dilutions to be determined by the researcher. Positive Control: CCRF-CEM, Jurkat or MOLT-4 cells. Paracortex in a tons il or a reactive lymph node. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Calc Crossreactivity :
Hu
Immunogen :
Interleukin-2-dependent human T lymphocytes
Specificity :
Recognizes human CD45RO. Species Crossreactivity: chimpanzee and common marmoset.
Purity :
Purified by Protein G affinity chromatography.
Form :
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138519.
Concentration :
~0.2mg/ml
Desc2 :
Product Type: Mab Isotype: IgG2a,k Clone No: UCHL-1 Host: mouse Source: human Concentration: ~0.2mg/ml Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138519. Purity: Purified by Protein G affinity chromatography. Immunogen: Interleukin-2-dependent human T lymphocytes Specificity: Recognizes human CD45RO. Species Crossreactivity: chimpanzee and common marmoset. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
E FC IF IHC IP WB
Storage Temperature :
-20°C
Reference :
1. Streuli M, Morimoto C, Schrieber M, Schlossman S, Saito H. J Immunol . 141:3910-3914(1988). 2. Tonks N, Charbonneau H, Diltz C, Fischer E and Walsh K. Biochem. 27:8695-8701(1988). 3. Ledbetter JA, Tonks NA, Fischer EH, Clark EA. Proc Natl Acad Sci USA 85:8628-8632(1988). 4. Knapp W, et al. Eds. Leucocyte Typing IV. Oxford University Press. New York (1989). 5. Thomas M. Annu. Rev. Immunol. 7:339 (1989). 6. Picker LJ, Treer JR, Ferguson-Darnell B, Collins PA, Buck D, Terstappen L. J Immunol . 150:1105-1121(1993). 7. Trowbridge I, et al.  Annu. Rev. Immunol. 12:85(1994). 8. Yamada M, et al. Stroke 27:1155 (1996). 9. Sakkas LI, et al. Clin. Diagn. Lab. Immunol. 5:430 (1998). 10. Ponsford M, et al. Clin. Exp. Immunol. 124:315 (2001). 11. D'Arena G, Cascavilla N, Nunziata G, et al. Leuk Lymphoma.43:1823-1825(2002).
Picture 1 File Name :
https://usbio-images.r.worldssl.net/prodimages/18/138519_1jpeg
company information
US Biological
4 Technology Way
Salem, MA01970
service@usbio.net
https://www.usbio.net
800-520-3011
headquarters: USA
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