This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
US Biological
product type :
antibody
product name :
CD45RA (Leukocyte Common Antigen, LCA)
catalog :
138517
quantity :
20 ug
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
158-4D3

The same clone is also sold as:
reactivity :
human
product information
Catalog Number :
138517
Product wo Prefix :
CD45RA (Leukocyte Common Antigen, LCA)
Host :
mouse
Product Type :
Mab
Category :
Antibodies
Size1 :
20 ug
Clone # USB :
158-4D3
Isotype :
IgG2a,k
Desc1 :
CD45 (leukocyte common antigen), the most common hematopoietic lineage marker, is a transmembrane glycoprotein tyrosine phosphatase with important roles in immune system signal transduction pathways. Antibody panels have been instrumental in identifying multiple isoforms, including CD45RA. Isoforms differ in their extracellular domain and are generated by alternative splicing of exons 4, 5 and 6 encoding peptide segments called A, B, and C, respectively. At least five different isoforms have been identified in humans: containing all three (ABC), two (AB and BC), one (B) or no (O) exons. A given antibody recognizes either every isoform (CD45 antibody) or only a subset (“restricted” CD45R antibody). The CD45RA antibody clone reacts with the ABC and BC isoforms (Schlossman et al, 1995). CD45RA has a molecular weight of 205-220kD. Isoform expression is regulated in lymphocyte type and activation-state dependent manners. CD45RA is expressed on subsets of T and B cells rendering the CD45RA antibody particularly useful for studying subpopulations. For example, naïve T lymphocytes express CD45RA containing the A exon which is lost after activation and replaced by CD45R0. In this regard, the CD45RA antibody is a useful naïve T cells marker since they express CD45RA, and activated or memory T cells are CD45RA negative and CD45RO positive (Schlossman et al, 1995). CD45RA is differentially expressed on lymphomas and the CD45RA antibody may also be used to help identify or classify lymphomas such as the CD45RA positive B from the CD45R0 positive T cell lymphomas. Researchers are encouraged to consult the scientific literature for additional information about CD45RA expression, including the use of antibody panels that include a CD45RA antibody for characterizing a given malignancy. Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence, Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug/10e6 cells Western Blot: 0.5-1ug/ml Immunofluorescence: 1-2ug/ml Immunoprecipitation: 1-2ug/500ug protein lysate Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT Optimal dilutions to be determined by the researcher. Positive Control: Daudi, Raji, IM9, and human lymphocytes. Human lymph nodes and tonsils. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Calc Crossreactivity :
Hu
Immunogen :
Stimulated human leukocytes
Specificity :
Recognizes human CD45RA.
Purity :
Purified by Protein G affinity chromatography.
Form :
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138517-AF.
Concentration :
~0.2mg/ml
Desc2 :
Product Type: Mab Isotype: IgG2a,k Clone No: 158-4D3 Host: mouse Source: human Concentration: ~0.2mg/ml Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138517AF. Purity: Purified by Protein G affinity chromatography. Immunogen: Stimulated human leukocytes Specificity: Recognizes human CD45RA. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
E FC IF IHC IP WB
Storage Temperature :
-20°C
Reference :
1. Shin H-M et al. Immune Network 11:114-122 (2011). 2. Clement LT. J Clin Immunol 12:1-10 (1992). 3. Saunders AE, P Johnson. Cellular Signaling 22:339-348 (2010) 4. Jacob MC et al. Am J Hematol 39:45-51 (1992).
Picture 1 File Name :
https://usbio-images.r.worldssl.net/prodimages/6/138517_1jpeg
company information
US Biological
4 Technology Way
Salem, MA01970
service@usbio.net
https://www.usbio.net
800-520-3011
headquarters: USA
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