CD43 (T Cell Marker, Galactoglycoprotein, GALGP, GPL115, Leukocyte sialoglycoprotein, Leukosialin, LSN, Sialophorin, SPN)

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

US Biological

product type :

antibody

product name :

CD43 (T Cell Marker, Galactoglycoprotein, GALGP, GPL115, Leukocyte sialoglycoprotein, Leukosialin, LSN, Sialophorin, SPN)

catalog :

138515

quantity :

20 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

DF-T1

The same clone is also sold as:

Santa Cruz Biotechnology: sc-6256
Dako: M0786
Novus Biologicals: NBP2-15190-0.1mg, NBP2-33140
Abnova: MAB6062, MAB6059, MAB6063, MAB6061, MAB11297, MAB6060
Invitrogen: MA5-16646, MA5-12194, MA5-28651
Bio-Rad: MCA555, MCA555F
Beckman Coulter: A89307, IM3264U

reactivity :

human

product information

Catalog Number :

138515

Product wo Prefix :

CD43 (T Cell Marker, Galactoglycoprotein, GALGP, GPL115, Leukocyte sialoglycoprotein, Leukosialin, LSN, Sialophorin, SPN)

Host :

mouse

Product Type :

Mab

Category :

Antibodies

Size1 :

20 ug

Clone # USB :

DF-T1

Isotype :

IgG1,k

Desc1 :

It recognizes a cell surface glycoprotein of 95/115/135kD (depending upon the extent of glycosylation), identified as CD43 [Workshop IV]. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma. Applications: Suitable for use in ELISA, Western Blot, Flow Cytometry, Immunoprecipitation, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug/10e6 cells Immunofluorescence: 1-2ug/ml Western Blot: 0.5-1ug/ml Immunoprecipitation: 1-2ug/500ug protein lysate Immunohistochemistry (Frozen & Formalin fixed paraffin embedded): 0.5-1ug/ml/30min @ RT Optimal dilutions to be determined by the researcher. Positive Control: Paracortex in a tonsil or a reactive lymph node Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Calc Crossreactivity :

Immunogen :

Myeloblastic KG1 cells

Specificity :

Recognizes human CD43.

Purity :

Purified by Protein A/G affinity chromatography.

Form :

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138515-AF.

Concentration :

~0.2mg/ml

Desc2 :

Product Type: Mab Isotype: IgG1,k Clone No: DF-T1 Host: mouse Source: human Concentration: ~0.2mg/ml Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 138515AF. Purity: Purified by Protein A/G affinity chromatography. Immunogen: Myeloblastic KG1 cells Specificity: Recognizes human CD43. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Calc Applications Abbrev :

E FC IF IHC IP WB

Storage Temperature :

-20°C

Reference :

1. Stross WP, et. al. Journal of Clinical Pathology, 1989, 42(9):953-61.

Picture 1 File Name :

https://usbio-images.r.worldssl.net/prodimages/44/138515_1jpeg