This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
US Biological
product type :
antibody
product name :
CD34 (Hematopoietic Progenitor Cell Antigen, HPCA1, Mucosialin) (PE)
catalog :
138512
quantity :
100Tests
clonality :
monoclonal
host :
mouse
conjugate :
PE
clone name :
ICO-115

The same clone is also sold as:
reactivity :
human
product information
Catalog Number :
138512
Product wo Prefix :
CD34 (Hematopoietic Progenitor Cell Antigen, HPCA1, Mucosialin) (PE)
Host :
mouse
Product Type :
Mab
Conjugate :
PE
Category :
Antibodies
Size1 :
100Tests
Clone # USB :
ICO-115
Isotype :
IgG1,k
Desc1 :
This antibody recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120kD, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Applications: Suitable for use in Flow Cytometry and Immunofluorescence. Other applications not tested. Recommended Dilutions: Flow Cytometry: 5ul/test to label 10e6cells or 5ul/100ul of whole blood Immunofluorescence: 1:50-1:100 Optimal dilutions to be determined by the researcher. Positive Control: KG-1 cells, tonsil or angiosarcoma Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Calc Crossreactivity :
Hu Rt
Immunogen :
Blast cells of a chronic myeloid leukemia patient. Cellular Localization: Cell surface
Specificity :
Recognizes human CD34. Species Crossreactivity: rat. Labels >85% of angiosarcoma and Kaposi's sarcoma, but shows low specificity.
Purity :
Purified by Protein A/G affinity chromatography.
Form :
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Labeled with R-Phycoerythrin (PE).
Concentration :
~0.1mg/ml
Desc2 :
Product Type: Mab Isotype: IgG1,k Clone No: ICO-115 Host: mouse Source: human Concentration: ~0.1mg/ml Form: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Labeled with R-Phycoerythrin (PE). Purity: Purified by Protein A/G affinity chromatography. Immunogen: Blast cells of a chronic myeloid leukemia patient. Cellular Localization: Cell surface Specificity: Recognizes human CD34. Species Crossreactivity: rat. Labels >85% of angiosarcoma and Kaposi's sarcoma, but shows low specificity. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Calc Applications Abbrev :
FC IF
Storage Temperature :
4°C Do Not Freeze
Reference :
1. Felshow DM et al. Blood 97:3768-3775 (2001). 2. Sato T et al. Blood 94:2548-2554 (1999).
Picture 1 File Name :
https://usbio-images.r.worldssl.net/prodimages/26/138512_1.jpg
company information
US Biological
4 Technology Way
Salem, MA01970
service@usbio.net
https://www.usbio.net
800-520-3011
headquarters: USA
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